Annexin 5 fluorescein isothiocyanate propidium iodide fitc pi apoptosis detection kit

PLGA75/25(1740)-PEG(1500)-PLGA75/25(1740) was purchased from MELOPEG (Shanghai, China). Gemcitabine hydrochloride (99%) was purchased from MedChemExpress (New Jersey, USA). Ethanol (98%) was purchased from SINOPHARM (Anhui, China). Polidocanol was purchased from GlpBio (California, USA). Cyanine 5.5 N-hydroxysuccinimide (Cy5.5 NHS) ester was purchased from APExBIO (Houston, USA). Dulbecco’s Modified Eagle Medium (DMEM) and fetal bovine serum (FBS) were from Gibco (Maryland, USA). Cell Counting Kit-8 (CCK-8) was purchased from Meilunbio (Dalian, China). Transwell Insert and Matrigel matrix were purchased from Corning (New York, USA). Annexin V-Fluorescein Isothiocyanate/Propidium Iodide (FITC/PI) apoptosis detection kit and Penicillin-Streptomycin-Amphotericin B liquid were purchased from Solarbio (Beijing, China). Terminal deoxynucleotidyl transferase dUTP Nick End Labeling (TUNEL) apoptosis kit was purchased from MULTI SCIENCES (Hangzhou, China). Proliferation marker protein Ki-67 was purchased from Servicebio (Wuhan, China).

After 48‐hour cultivation in an incubator, transfected H23 and H1299 cells were collected and resuspended with 1 × binding buffer. An Annexin V‐fluorescein isothiocyanate/propidium iodide (FITC/PI) apoptosis detection kit (Solarbio, Beijing, China) was selected to evaluate the apoptosis of H23 and H1299 cells. The cell suspension was then added to Annexin V‐FITC and PI solution for 10‐minutes incubation. A flow cytometer (BD Biosciences, San Jose, CA, USA) was then used to analyze the apoptotic cells.