Fm4 64 styryl dye

Manufactured by Thermo Fisher Scientific

FM4-64 styryl dye is a fluorescent membrane stain used in biological research. It is a lipophilic dye that selectively labels the plasma membrane of cells. The dye can be used to visualize and study cellular membrane dynamics, endocytosis, and other membrane-related processes.

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Lab products found in correlation

Csu w1 t3, by Yokogawa (2 mentions) Axio observer, by Zeiss (2 mentions)

2 protocols using fm4 64 styryl dye

Visualizing Synaptic Vesicle Dynamics

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Neurons were seeded into microfluidic devices and transduced at DIV1 with GFP lentivirus. The synaptic chamber was incubated at indicated time with 10 µM of FM4-64 styryl dye (ThermoFischer Scientific) into high KCl Tyrode solution (2 mM NaCl; 50 mM KCl; 2 mM CaCl₂; 1 mM MgCl₂; 10 mM Glucose and 1 mM Hepes buffer pH7.4) during 1 min at 37°C. After three washes with Tyrode solution (150 mM NaCl; 4 mM KCl; 2 mM CaCl₂; 1 mM MgCl₂; 10 mM Glucose and 1 mM Hepes buffer pH7.4) containing inhibitors of additional firing (1 mM kynurenic acid and 10 mM MgCl₂), acquisitions were made on inverted microscope (Axio Observer, Zeiss) coupled to a spinning-disk confocal system (CSU-W1-T3; Yokogawa) connected to an electron-multiplying CCD (charge-coupled device) camera (ProEM+1024, Princeton Instrument) at 37°C and 5% CO2 with z stacks of 5 µm.

Bruyère J., Abada Y.S., Vitet H., Fontaine G., Deloulme J.C., Cès A., Denarier E., Pernet-Gallay K., Andrieux A., Humbert S., Potier M.C., Delatour B, & Saudou F. (2020). Presynaptic APP levels and synaptic homeostasis are regulated by Akt phosphorylation of huntingtin. eLife, 9, e56371.

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Synaptic Vesicle Dynamics Monitoring

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Neurons were seeded into microfluidic devices and transduced at DIV1 with GFP lentivirus. The synaptic chamber was incubated at indicated time with 10 µM of FM4-64 styryl dye (ThermoFischer Scientific) into high KCl Tyrode solution (2 mM NaCl; 50 mM KCl; 2 mM CaCl 2 ; 1 mM MgCl 2 ; 10 mM Glucose and 1 mM Hepes buffer pH7.4) during 1 min at 37°C. After 3 washes with Tyrode solution (150 mM NaCl; 4 mM KCl; 2 mM CaCl 2 ; 1 mM MgCl 2 ; 10 mM Glucose and 1 mM Hepes buffer pH7.4) containing inhibitors of additional firing (1 mM kynurenic acid and 10 mM MgCl 2 ), acquisitions were made on inverted microscope (Axio Observer, Zeiss) coupled to a spinning-disk confocal system (CSU-W1-T3; Yokogawa) connected to an electron-multiplying CCD (charge-coupled device) camera (ProEM+1024, Princeton Instrument) at 37°C and 5% CO2 with z stacks of 5 µm.

Bruyère J., Abada Y., Vitet H., Fontaine G., Deloulme J., Cès A., Denarier É., Pernet‐Gallay K., Andrieux A., Humbert S., Potier M., Delzescaux T., & Saudou F. (2020). Presynaptic APP levels and synaptic homeostasis are regulated by Akt phosphorylation of Huntingtin.

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