Axion vision rel 4

Manufactured by Zeiss

Sourced in Germany

The Axion Vision Rel.4.6 is a software solution developed by Zeiss for the analysis and processing of microscopic images. It provides a range of tools and functionalities to enhance the quality and productivity of microscopy workflows.

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Lab products found in correlation

Anti γh2ax antibody, by Cell Signaling Technology (2 mentions) Chamber slide cultures, by Thermo Fisher Scientific (1 mentions) Anti ddup antibody, by Sino Biological (1 mentions) Anti pcna, by Cell Signaling Technology (1 mentions) Phenol red free medium, by Thermo Fisher Scientific (1 mentions) Bromodeoxyuridine (brdu), by Roche (1 mentions) Glass bottom plates, by NEST Biotechnology (1 mentions) Anti cleaved caspase 3 clone 5a1e, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Rel 4.8 (8 citations) Axion Vision 4.8 software (3 citations)

3 protocols using axion vision rel 4

Antibody Imaging Analysis in Cell Cultures

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The indicated cells were plated on chamber slide cultures (Thermo Fisher Scientific, CA, USA), and then treated with anti-DDUP antibody (Sino Biological, Wuhan, China), anti-γH2AX antibody (#9718, Cell Signaling Technology, MA, USA), anti-PCNA (#2586, Cell Signaling Technology, MA, USA), anti-RAD18 antibody (#9040, Cell Signaling Technology, MA, USA), or anti-RAD51C antibody (PA5-77078, Invitrogen, USA). The photographs were taken with the Axion Vision Rel.4.6 computerized image analysis system (Carl Zeiss, Jena, Germany).

Ren L., Qing X., Wei J., Mo H., Liu Y., Zhi Y., Lu W., Zheng M., Zhang W., Chen Y., Zhang Y., Pan T., Zhong Q., Li R., Zhang X., Ruan X., Yu R, & Li J. (2023). The DDUP protein encoded by the DNA damage-induced CTBP1-DT lncRNA confers cisplatin resistance in ovarian cancer. Cell Death & Disease, 14(8), 568.

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Local UV Laser Micro-irradiation of HeLa Cells

Cited 1 time

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HeLa cells were seeded on 15 mm diameter glass-bottom plates (NEST, Wuxi, China) and presensitized with 10 μM Brdu (Roche, Basel, Switzerland) in phenol red-free medium (Invitrogen, USA) for 24 h at 37°C. Local laser micro-irradiation was performed by a micropoint system (Andor, Belfast, UK) with a 365 nm pulsed nitrogen UV laser (16 Hz pulse, 55% laser output). After irradiation, cells were immunostained as previously described (27 (link)) with anti-γH2AX antibody (#9718, Cell Signaling Technology). The images were captured using the Axion Vision Rel.4.6 computerized image analysis system (Carl Zeiss, Jena, Germany).

Yu R., Hu Y., Zhang S., Li X., Tang M., Yang M., Wu X., Li Z., Liao X., Xu Y., Li M., Chen S., Qian W., Gong L.Y., Song L, & Li J. (2022). LncRNA CTBP1-DT-encoded microprotein DDUP sustains DNA damage response signalling to trigger dual DNA repair mechanisms. Nucleic Acids Research, 50(14), 8060-8079.

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Quantifying Apoptosis in Rabbit Tissue

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It was used rabbit monoclonal antibody anti-cleaved caspase-3, clone 5A1E -Cell Signaling Technology®. The number of apoptotic bodies was counted by the pathologist, without prior knowledge of the group to which each animal belonged. The scale for the quantification of the apoptotic corpuscles was done as described previously All images and measurements were obtained using the Axion Vision REL 4.6 -Carl Zeiss.

Gomes R.O., Artigiani R N.e.t.o., Guimarães JF N.e.t.o., Nunes A.P., Montero E.F, & Martins J.L. (2017). Neonatal necrotizing enterocolitis rat model attenuated by a remote ischemic preconditioning in the pregnant. Acta cirurgica brasileira, 32(3).

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