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Apc labeled anti cd14

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APC-labeled anti-CD14 is a fluorescent antibody used for the detection and identification of CD14-expressing cells by flow cytometry. CD14 is a glycosylphosphatidylinositol (GPI)-anchored protein expressed on the surface of monocytes, macrophages, and granulocytes. The APC (Allophycocyanin) fluorescent label allows for the visualization and quantification of CD14-positive cells in a sample.

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Lab products found in correlation

Pe labeled anti cd11b, by BioLegend (1 mentions) Ara c, by Selleck Chemicals (1 mentions) Canto plus, by BD (1 mentions)

Close spelling variants of this product

CD14-APC (31 citations) Anti-CD14-APC (13 citations)

2 protocols using apc labeled anti cd14

1

Cell Differentiation Assay with FTO Knockdown

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For in vitro cell differentiation assay, FTO knockdown and control cells were incubated with 250 ng/μl Ara-C (#S1648, Selleck) for 48 h, cells were harvested and washed with chilled PBS and stained with PE-labeled anti-CD11b (#301306, BioLegend, California, USA) and APC-labeled anti-CD14 (#367118, Biolegend) for flow cytometry analysis. The fluorescence intensity was detected using CANTO PLUS (BD Biosciences, New Jersey, USA) and analyzed with the FlowJo.
Zhang Z.W., Zhao X.S., Guo H, & Huang X.J. (2023). The role of m6A demethylase FTO in chemotherapy resistance mediating acute myeloid leukemia relapse. Cell Death Discovery, 9, 225.
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2

Whole Blood Flow Cytometry Analysis

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Cells surface staining were performed on whole blood using allophycocyanin (APC)-labeled anti-CD14 (Biolegend, CA, USA), phycoerythrin (PE)-labeled anti-Flt-1 (Santa Cruz, CA, USA) monoclonal antibodies (mAbs). Red cells were lysed before the analysis. After twice washes with PBS, the cells were examined by the flow cytometry (FACSSCalibur, Becton Dickinson), and the data were analyzed with FlowJo V10.8.1 (FlowJo, Ashland, OR, USA).
Cheng W., Wang L., Gao X., Yin G., Wang J., Shu Y., Qiu H, & Duan L. (2023). Clinical value of soluble fms-like tyrosine kinase 1 (sFlt-1) in adult secondary hemophagocytic lymphohistiocytosis. Journal of the Chinese Medical Association : JCMA, 86(7).
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