Cell migration assays were performed in 8-µm pore size Transwell filter chamber inserts (Corning, USA). Cells were diluted 1:1 in 0.2% trypan blue and counted in duplicate using an Olympus microscope (Tokyo, Japan). For migration assays, 5×10^4 cells in 200 µl DMEM without FBS medium were suspended into the upper chamber and 800 µl DMEM supplemented with 10% FBS was added in the lower chamber per well. After incubation for 14 h at 37°C and 5% CO2, cells moved to the bottom surface of the membrane were fixed with 100% methanol for 20 min and stained with 0.1% crystal violet for 10 min and washed with water for three times. Then, the invading cells were counted and imaged in at least 10 random fields under a light microscope (Olympus, Japan). The assays were performed three times independently.
Transwell filter chamber inserts
Manufactured by Corning
Sourced in United States
Transwell filter chamber inserts are a laboratory equipment product designed for cell culture applications. They consist of a permeable membrane support that allows for the separation of cells or cell layers in a culture system. The inserts facilitate the study of cell-cell interactions, cell migration, and other cellular processes by creating a barrier between different compartments within the culture environment.
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Lab products found in correlation
2 protocols using transwell filter chamber inserts
1
Cell Migration Assay Protocol
2
Transwell Assay for Cell Migration and Invasion
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We put the 8 μm pore size Transwell filter chamber inserts (Corning, NY, USA) to work by examining cell migration and invasion assay. After transfected with the indicated virus, 2 × 104 cells were respectively seeded into transwell chambers. To fuel the migration or invasion, we added the RPMI-1640 containing 10% FBS to the lower chamber. For 24 h at 37 ° C and 5% CO2, we incubated the migrated cells. Then, we fixed the migrated cells in 4% paraformaldehyde and stained them with 0.1% crystal violet and imaged before taking an image.
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