Anti sdha

Hippocampal membrane-associated proteins from 8-week-old male C57BL/6 mice treated with Ketamine for 2 h (Ketamine-treated animals: n=4, vehicle-treated animals: n=5), 14 h (Ketamine-treated animals: n=5, vehicle-treated animals: n=4), 24 h (Ketamine-treated animals: n=5, vehicle-treated animals: n=5) and 72 h (Ketamine-treated animals: n=5, vehicle-treated animals: n=3) were fractionated by SDS-polyacrylamide gel electrophoresis with 12% separating gels and western blotting was performed based on standard protocols. After electrophoresis, proteins were transferred to polyvinylidene difluoride membranes (Immobilon-P, Millipore, Billerica, MA, USA). The primary antibody was against the subunit A of the succinate dehydrogenase complex (Anti-SDHA, 1:200, sc98253, Santa Cruz Biotechnology, Dallas, TX, USA). An anti-rabbit ECL horseradish peroxidase-linked secondary antibody (1:10 000; NA934, GE Healthcare Life Sciences, Little Chalfont, Buckinghamshire, UK) was used. The densitometric analyses were performed with the Image Lab software (Bio-Rad Laboratories, Munich, Germany).

Unless stated otherwise, all chemicals were purchased from Sigma-Aldrich or ThermoFisher Scientific. The following antibodies were used: anti-β-actin (Novus Biologicals, Cat# NB600-501), anti-ATP5A (Abcam, clone 15H4C4, Cat# ab14748), anti-COX4 (Novus Biologicals, Cat# NB110-39115), anti-GAPDH (Protein Tech, Cat# 60004–1-Ig), anti-HSP60 (Santa Cruz, clone B-9, Cat# sc-271215 or Protein Tech, Cat# 15282–1-AP), anti-IQGAP1 (Santa Cruz, clone D-3, Cat# sc-374307 or clone C-9, Cat# sc-376021), anti-Lamin B1 (Santa Cruz, clone A-11, Cat# sc-377000), anti-Myc-Tag (Cell Signaling, clone 9B11, Cat#2276), anti-NDUFS4 (Novus Biologicals, Cat# NBP1-89026-25), anti-NDUFA4 (Santa Cruz, clone 2G7, Cat# sc-517091), anti-NDUFS1 (Santa Cruz, clone E-8, Cat# sc-271510), anti-NDUFAF2 (mimitin) (Santa Cruz, clone H-11, Cat# sc-365592), anti-SDHA (Santa Cruz, clone F-2, Cat# sc-390381), anti-UQCRC2 (Santa Cruz, clone G-10, Cat# sc-390378), anti-VDAC2 (Protein Tech, Cat# 11663-I-AP), HRP-conjugated goat anti-rabbit IgG (SouthernBiotech, Cat# 4050–05), HRP-conjugated goat anti-mouse IgG (SouthernBiotech, Cat# 1030–05), anti-rabbit-Alexa Fluor 555 (Molecular Probes, Cat# A27039), anti-rabbit-Alexa Fluor 594 (Molecular Probes, Cat# A21207), anti-mouse Alexa Fluor 488 (Molecular Probes Cat# A28175).