Polyclonal antibodies to vimentin

Manufactured by Cell Signaling Technology

Sourced in United States

Polyclonal antibodies to vimentin are a type of laboratory reagent produced by Cell Signaling Technology. Vimentin is an intermediate filament protein found in various cell types. These antibodies can be used for the detection and study of vimentin in biological samples.

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Lab products found in correlation

2 protocols using polyclonal antibodies to vimentin

Epithelial-Mesenchymal Transition Signaling Assay

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Cell-culture reagents were from Gibco (Grand Island, NY, USA). Lipofectamine and TRIzol were from Invitrogen (Carlsbad, CA, USA). G418 was from Merck (Darmstadt, Germany). Monoclonal antibody to IGFBP-rP1 and recombinant human IGFBP-rP1 protein were from RD (Minneapolis, MN, USA). Mouse monoclonal antibodies to E-cadherin, N-cadherin, TGF-β R I, TGF-β R II, β-catenin, actin, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Polyclonal antibodies to vimentin, MMP9, Snail, Smad2, P-Smad2, Smad3, and P-Smad3 were from Cell Signaling (Danvers, MA, USA). Recombinant human TGF-β1 was from Peprotech (Rocky Hill, NJ, USA). SB431542 (TGF-β/ALK5/Smad2 inhibitor) was obtained from Sigma.

Zhu S., Zhang J., Xu F., Xu E., Ruan W., Ma Y., Huang Q, & Lai M. (2015). IGFBP-rP1 suppresses epithelial–mesenchymal transition and metastasis in colorectal cancer. Cell Death & Disease, 6(3), e1695-.

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Protein Expression and Antibody Reagents

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Human recombinant TGFβ1 was purchased from R&D Systems (Minneapolis, MN). Monoclonal antibody to E-cadherin was obtained from BD Biosciences, and monoclonal antibody to ZO-1 was from Invitrogen (Carlsbad, CA). Monoclonal antibody against glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and polyclonal antibody against PKCε and Twist were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Polyclonal antibodies to vimentin, claudin-1, cleaved caspase-3, and cleaved caspase-7 were from Cell Signaling Technology (Danvers, MA). Monoclonal antibody against β-actin was purchased from Sigma (St. Louis, MO). Horseradish peroxidase-conjugated goat anti-mouse and donkey anti-rabbit antibodies were purchased from Jackson Immuno Research Lab Inc (Bar Harbor, ME). Alexa Fluor® 488 goat anti-mouse and Alexa Fluor® 568 goat anti-rabbit antibodies were from Invitrogen (Carlsbad, CA), and 4′,6-diamidino-2-phenylindole (DAPI) was purchased from Molecular Probes (Eugene, OR). ON-TARGETplus control and PKCε siRNAs were purchased from Dharmacon (Lafayette, CO). Polyvinylidene difluoride membrane was obtained from Millipore (Billerica, MA). Enhanced chemiluminescence detection kit was from Amersham.

Jain K, & Basu A. (2014). Protein Kinase C-ε Promotes EMT in Breast Cancer. Breast Cancer : Basic and Clinical Research, 8, 61-67.

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