Dephosphorylated myelin basic protein

Manufactured by Merck Group

Dephosphorylated myelin basic protein (MBP) is a laboratory reagent derived from myelin, the protective sheath around nerve fibers. MBP is a structural protein that plays a crucial role in the formation and maintenance of the myelin sheath. The dephosphorylated form of MBP is commonly used in research applications to study the properties and functions of this important protein.

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Lab products found in correlation

Gf109203x, by Merck Group (2 mentions) Pyrogard filter, by Merck Group (2 mentions) Pkc lipid activator cocktail, by Merck Group (2 mentions) Polyclonal phospho ser pkc substrate antibody, by Cell Signaling Technology (2 mentions) Adenosine 5 γ thio triphosphate, by Merck Group (1 mentions) 3 mercaptopropyl triethoxysilane, by Merck Group (1 mentions) Horseradish peroxidase hrp, by Merck Group (1 mentions) Adenosine triphosphate (atp), by Merck Group (1 mentions)

2 protocols using dephosphorylated myelin basic protein

Quantitative Protein Kinase C Activity Assay

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All chemicals were of analytical grade and were used as received, unless otherwise specified. All aqueous solutions were made with double de-ionised water, 18.2 MΩ cm, with a Pyrogard filter (Millipore, USA). Tris base, MgCl₂, NaCl, acetone, NH₄OH, HCl, H₂O₂, 3-glycidoxypropyltrimethoxysilane (GOPTS), PKC-α kinase inhibitor GF 109203X, adenosine tri-phosphate (ATP), adenosine 5'-[γ-thio] triphosphate (ATP-S), 3-mercaptopropyl triethoxysilane (MPTES), 3,3′,5,5′-tetramentylbenzidine (TMB), allyl hydroxyl silane (AHS) and horseradish peroxidase (HRP) were purchased from Sigma-Aldrich. Dephosphorylated myelin basic protein (MBP), purified from bovine brain using FPLC liquid chromatography, was purchased from Millipore. Protein Kinase C alpha (PKC-α) human recombinant kinase produced in Sf9, was procured from ProSec-Tray TechnoGene Ltd. PKC lipid activator cocktail was obtained from Millipore. Polyclonal phospho-(Ser) PKC substrate antibody was purchased from Cell Signaling Technology. 20 nm research grade gold nanoparticles (AuNPs) were bought in colloidal form from Diagnostic Consulting Network.

Bhalla N., Di Lorenzo M., Pula G, & Estrela P. (2015). Protein phosphorylation detection using dual-mode field-effect devices and nanoplasmonic sensors. Scientific Reports, 5, 8687.

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Characterization of PKC-α Kinase Activity

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All chemicals were of analytical grade and used as received, unless otherwise specified. All aqueous solutions were made with ionised water, 18.2 MΩ cm, with a Pyrogard filter (Millipore, USA). Tris base, magnesium chloride, sodium chloride, acetone, ammonium hydroxide, hydrochloric acid, hydrogen peroxide, mercaptoundecanoic acid (MUA), mercaptohexanol (MCH), ethanolamine, ethanol, NHS (N-hydroxysuccinimide) , EDC (ethyl-dimethylaminopropyl carbodiimide), PKC-α kinase inhibitor GF 109203X, adenosine tri-phosphate (ATP) Gold nanoparticles, 20 nm average spherical diameter, in 0.1 mM PBS were purchased from Sigma-Aldrich. Dephosphorylated myelin basic protein (MBP), purified from bovine brain using fast protein liquid chromatography (FPLC), was purchased from Millipore. PKC-α human recombinant kinase produced in Sf9, was procured from ProSec-Tray TechnoGene Ltd. PKC lipid activator cocktail was obtained from Millipore. Polyclonal phospho-(Ser) PKC substrate antibody was purchased from Cell Signaling Technology.

Bhalla N., Formisano N., Miodek A., Jain A., Di Lorenzo M., Pula G, & Estrela P. (2015). Plasmonic ruler on field-effect devices for kinase drug discovery applications. Biosensors & bioelectronics, 71.

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