Human lung and mouse lung tissues were stained with rabbit monoclonal MBD2 antibody (1:500, Abcam, Cambridge, UK) and rabbit polyclonal MBD2 antibody (1:100, Santa Cruz Biotechnology, CA, USA), respectively. These proteins were then detected in lung sections with an IgG Streptavidin Biotin Complex kit (Boster, Wuhan, People’s Republic of China) and developed with DAB substrate according to the manufacturer’s protocol (Dako, Glostrup, Denmark). A Nikon Spot image acquisition and processing system (USA) was used for image assessment. The mean integral OD of MBD2 protein staining in the bronchiolar epithelium was measured as follows.32 (link) We used Image-Pro Plus 4.1 professional image analysis software to open all immunohistochemical images from one sample slide. Thereafter, we activated the Magnetic Lasso tool and dragged selection areas only containing bronchial epithelium, then copy and pasted it into a new image. Then, the area of bronchial epithelium and the integral OD of MBD2-positive epithelium were measured. Finally, the mean integral OD of MBD2 protein staining in the bronchial epithelium was the ratio of the integral OD of MBD2-positive epithelium to area of bronchial epithelium. All of the images are assessed with the same parameters.
Spot image acquisition and processing system
Manufactured by Nikon
Sourced in United States
The Spot image acquisition and processing system is a versatile tool for capturing and analyzing high-quality digital images. It enables users to acquire, process, and manage images with precision and efficiency. The system provides core functionalities for image data capture and processing, catering to the needs of various applications.
Automatically generated - may contain errors
4 protocols using spot image acquisition and processing system
1
Quantifying MBD2 Protein in Lung Tissues
2
Siglec-9 Immunostaining of Lung Sections
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The lung sections were stained with anti-Siglec-9 antibody (R&D Systems, Minneapolis, USA) using a modified method as previously described52 (link). A Nikon Spot image acquisition and processing system was used for image assessment.
3
MTMR14 Expression in Lung Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Anti-MTMR14 antibody (ABclonal) was used to stain the paraffin-embedded lung tissue sections. The expression of MTMR14 in lung sections was detected using an IgG Streptavidin Biotin Complex kit (Boster). The staining was developed with DAB substrate (Dako, Glostrup, Denmark) and observed using a Nikon Spot image acquisition and processing system (USA).
4
Immunohistochemical Analysis of STIM1 in Lung Tissue
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Formalin (10%)-fixed, paraffin-embedded lung tissue sections of healthy non-smokers (HN), smokers without COPD, and smokers with COPD (SC) were deparaffinized using xylene and rehydrated in a graded ethanol series. Heat-induced antigen retrieval was performed using a microwave. After cooling with running tap water, sections were incubated with 3% hydrogen peroxide to block endogenous peroxidase activity, followed by 1-h incubation in 5% BSA-phosphate-buffered saline (PBS) solution at room temperature to avoid non-specific background. Then, slides were incubated with polyclonal rabbit anti-STIM1 (1:500; Proteintech, United States) antibody at 4°C overnight in a humidified chamber. After washing, sections were incubated with a peroxidase-conjugated goat anti-rabbit secondary antibody for 1 h at room temperature. The reactions were developed using a DAB substrate kit with hematoxylin as a counterstain. A Nikon Spot image acquisition and processing system (United States) was used for image assessment.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!