MOG35-55 used to induce EAE in the mice was purchased from ProSpec-Tany TechnoGene, Ltd. Freund's complete adjuvant (CFA) containing Mycobacterium tuberculosis (M. tuberculosis) strain H37Rv was obtained from BD Biosciences. Pertussis toxin (PTX) was obtained from List Biological Labs. Mouse allophycocyanin (APC)-conjugated IL-17antibody (cat. no. 506916; cloneTC11-18H10), mouse fluorescein isothiocyanate (FITC)-conjugated interferon (IFN)-γ antibody (cat. no. 505805; clone XMG1.2), mouse PE-Cy7-conjugated CD4 antibody (cat. no. 100528; clone RM4.5), mouse Alexa Fluor@ 488-conjugated Foxp3 (cat. no. 320012; clone MF23) antibody, mouse FITC-conjugated anti-mGr.1 (cat. no 108405; clone RB6-8C5, BD), phycoerythrin (PE)-anti-mCD11b (cat. no. 101255; clone M1/7), mouse FITC-anti-mCD8a (cat. no. 100705; clone 53-6.7), PE-anti-mB220 (cat. no. 103207; clone RA3-682), PE-Cy7-anti-mCD4 (clone GK.1.5), mouse APC-conjugated CD3 (cat. no 100312; clone 145-2C11) were purchased from BioLegend, Inc. ELISA kits for IL-17A (cat. no. 88-7371), IgG (cat. no. 88-50400), IgG1 (cat. no. 88-50410), IgG2a (cat. no. 88-50420) and IgG3 (cat. no. 88-50440) were purchased eBioscience. Reverse transcription kit and SYBR Premix Ex Taq II were purchased from Takara Biotechnology Co., Ltd. RNA extraction kit was purchased from Qiagen, Inc. All primers used were synthesized by Shanghai Bioengineering Co., Ltd.
Elisa kits for il 17a
Manufactured by BioLegend
BioLegend's ELISA kits for IL-17A are designed to quantitatively measure the levels of this cytokine in biological samples. These kits utilize the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify IL-17A.
Automatically generated - may contain errors
2 protocols using elisa kits for il 17a
1
Experimental autoimmune encephalomyelitis induction
2
Cytokine Profiling of Primary Cells and Biological Fluids
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Primary cell culture supernatants were analyzed using ELISA kits for IL‐17A (Biolegend or eBioscience), IL‐17F (eBioscience), IL‐2, or IFN‐γ (both from Biolegend). In some cases, cytokine levels were determined by luminex assay (Bio‐Plex ProTM Human Th17 Cytokine Assays, Bio‐Rad Laboratories, CA, USA). Fibroblast culture supernatants were analyzed using standard IL‐6 and IL‐8 Biolegend ELISA kits and a luminex assay (R&D) for CXCL1, CXCL5, and CCL20 levels. Cell‐free serum and synovial fluid samples were analyzed using Platinum kits for IL‐17A and IL‐17F (eBioscience) and a luminex assay (Bio‐Plex ProTM Human Th17 Cytokine Assays, Bio‐Rad Laboratories) for IL‐1β and IL‐23. All analyses were performed according to manufacturer's instructions.
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