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Real time system

Manufactured by Agilent Technologies
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The Real-time System by Agilent Technologies is a laboratory equipment designed for real-time data acquisition and analysis. It provides a platform for monitoring and recording various experimental parameters in real-time.

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Lab products found in correlation

Trizol reagent, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

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2 protocols using real time system

1

BM-MSCs and Mandible RNA Extraction

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Total RNA was extracted from the cultured BM-MSCs and mandibles using Trizol reagent (Invitrogen) according to the manufacturer's instructions. Complementary DNA (cDNA) was synthesized using Synthesis SuperMix (Invitrogen). Real-time RT-PCR was carried out using an Agilent Real-time System. Gapdh was amplified at the same time to normalize gene expression. Each experiment was repeated three times to determine relative gene expression differences. The sequence-specific primers of human and mice are displayed in Table 1.
Chen H., Hu X., Yang R., Wu G., Tan Q., Goltzman D, & Miao D. (2020). SIRT1/FOXO3a axis plays an important role in the prevention of mandibular bone loss induced by 1,25(OH)2D deficiency. International Journal of Biological Sciences, 16(14), 2712-2726.
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2

Quantitative Gene Expression Analysis in MSCs

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Total RNA was extracted from cultured MSCs and lumbar vertebrae using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. Complementary DNA (cDNA) was synthesized using Synthesis SuperMix (Invitrogen). The real‐time RT–PCR was carried out using an Agilent Real‐time System. Gapdh was amplified at the same time to normalize gene expression. Groups at least six mice were examined, and each experiment was repeated three times to determine relative gene expression differences. The PCR primer sequences used in this study are shown in the Table S2.
Yang R., Chen J., Zhang J., Qin R., Wang R., Qiu Y., Mao Z., Goltzman D, & Miao D. (2019). 1,25‐Dihydroxyvitamin D protects against age‐related osteoporosis by a novel VDR‐Ezh2‐p16 signal axis. Aging Cell, 19(2), e13095.
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