The nZnO (10–30 nm 99% purity) was purchased from Nanosany Company (US-Nano), Mashhad, Iran. The NPs were fully characterized using transmission electron microscopy (TEM) (100 kV Philips, EM208, Sigma-Aldrich, Germany) and scanning electron microscopy (SEM) (JSM-840A, JEOL, Tokyo, Japan) images. Briefly, the specific surface area ranged from 20 to 60 m2 g–1 with a density of 5.6 g/cm3. A detailed analysis of NPs physiochemical properties is provided in Supplementary Figure 1 . Zinc sulfate (ZnSO4.7H2O) was supplied by Sigma-Aldrich (Milan, Italy). The formaldehyde, glacial acetic acid, ethanol, acetocarmine, acid fuchsin, and orange G used at analytical grade were purchased from Sigma-Aldrich (Heidelberg, Germany).
Zinc sulfate znso4.7h2o
Manufactured by Merck Group
Sourced in Italy, Germany, Czechia
Zinc sulfate (ZnSO4.7H2O) is a white crystalline compound that contains the essential mineral zinc. It is a commonly used laboratory reagent with the chemical formula ZnSO4·7H2O. The compound is soluble in water and has various applications in scientific research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Glacial acetic acid, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Formaldehyde, by Merck Group (1 mentions)
Acid fuchsin, by Merck Group (1 mentions)
Jsm 840a, by JEOL (1 mentions)
Orange g, by Merck Group (1 mentions)
Acetocarmine, by Merck Group (1 mentions)
Nutrient broth, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Lonza (1 mentions)
Amphotericin b, by Lonza (1 mentions)
Streptomycin sulfate, by Lonza (1 mentions)
Penicillin g sodium, by Lonza (1 mentions)
L glutamine solution, by Lonza (1 mentions)
Mitotracker green fm, by Thermo Fisher Scientific (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Mitosox red reagent, by Thermo Fisher Scientific (1 mentions)
Triton x, by Merck Group (1 mentions)
Bafilomycin a1, by Merck Group (1 mentions)
Newport green dcf diacetate, by Thermo Fisher Scientific (1 mentions)
Monodansylcadaverine, by Merck Group (1 mentions)
3 protocols using zinc sulfate znso4.7h2o
1
Characterization of Nanoparticle Properties
2
Cytotoxicity Evaluation of Zinc Sulfate
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Zinc sulfate (ZnSO4·7H2O) was purchased from Sigma, Aldrich, Germany, and was used for zinc solution preparation. Nutrient broth and agar medium were purchased from Oxoid, UK. 3-(4, 5-dimethyIthiazol-2-y1)-2-5-diphenyl tetrazolium bromide (MTT) was obtained from Merck KGaA (Darmstadt, Germany). 10% fetal bovine serum (FBS), 2 mM L-glutamine solution, 100 units/ml penicillin G sodium, 100 units/ml streptomycin sulfate, and 250 g/ml amphotericin B were obtained from Lonza, Basel, Switzerland. All chemicals were analytical grades.
3
Zinc Sulfate Effects on Cellular Processes
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Zinc sulfate (ZnSO 4 . 7 H 2 O) (Sigma-Aldrich, Czech Republic) was dissolved in a serum-free medium, sterilized by ultrafiltration, and stored until use as a stock solution of 1M in a refrigerator (4°C). The cells were treated with a range of zinc sulfate concentrations of for up to 72 h.
The dyes JC-1, acridine orange 10-nonyl bromide (NAO), Newport Green™ DCF diacetate, MitoSOX™ Red reagent, DQ-Green BSA and MitoTracker Green FM were acquired from Molecular Probes, Inc. (Eugene, U.S.A.). Triton-X; BrdU; monodansylcadaverine (MDC); 4´,6-diamidino-2-phenylindole (DAPI) and Bafilomycin A1 were obtained from Sigma-Aldrich (St. Louis, MO, USA). Monoclonal and polyclonal antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), secondary antibodies from Alexis Corporation (Lausen, Switzerland) and Dako (Glostrup, Denmark). All other chemicals were of the highest analytical grade.
The dyes JC-1, acridine orange 10-nonyl bromide (NAO), Newport Green™ DCF diacetate, MitoSOX™ Red reagent, DQ-Green BSA and MitoTracker Green FM were acquired from Molecular Probes, Inc. (Eugene, U.S.A.). Triton-X; BrdU; monodansylcadaverine (MDC); 4´,6-diamidino-2-phenylindole (DAPI) and Bafilomycin A1 were obtained from Sigma-Aldrich (St. Louis, MO, USA). Monoclonal and polyclonal antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA), secondary antibodies from Alexis Corporation (Lausen, Switzerland) and Dako (Glostrup, Denmark). All other chemicals were of the highest analytical grade.
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