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2 protocols using tetrahydrofurane thf

1

Enzymatic Dehalogenation of Chlorinated Solvents

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Thermomyces lanuginosus solution (Lipozyme® TL 100L) was from Novozymes (Copenhagen, Denmark). Copper (II) sulfate pentahydrate, sodium acetate and hydrogen peroxide (33%, H2O2) were from Panreac (Barcelona, Spain). Graphite flakes, sodium bicarbonate, sodium phosphate, sodium borohydride, Rhodamine B (RhB), trichloroethylene (TCE), 1,1-dichloroethylene (1,1-DCE), and vinyl chloride (VC) were purchased from Merck (Darmstadt, Germany). HPLC grade acetonitrile, methanol, tetrahydrofurane (THF) and dioxane (98%) were from Scharlau (Barcelona, Spain). Tween-80, dodecyl sulphate sodium (85%), Mercaptoethanol and cetyl trimethyl ammonium bromide (CTAB) were purchased from Thermo Fischer Scientific (Waltham, MA, USA).
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2

Patulin Quantification in Food Samples

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Patulin pure standard and dibasic potassium phosphate trihydrated were purchased from Sigma-Aldrich (Sant Louis, MO, USA). Sodium hydroxide and hydrochloric acid solutions, sodium carbonate, anhydrous sodium sulphate and citric acid from Panreac (Barcelona, Spain). Formic acid, malic acid and ferrous sulphate heptahydrate from VWR (Fontenay-sous-Bois, France). Acetic acid, methanol HPLC grade, ethyl acetate and sucrose from Fisher Scientific (Loughborough, UK). Tetrahydrofurane (THF) from Scharlau (Barcelona, Spain) and GSH from Across Chemicals (Geel, Belgium). Milli-Q water was obtained from a Millipore system (Milford, USA).
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