Firefly and Renilla luciferases were measured sequentially using the Dual-Luciferase reporter assay system (Promega) according to the manufacturer’s protocol. After 24 h of retinoid treatment, the medium was removed and the cells were washed with 250 μl of PBS; 100 μl of passive lysis buffer was then added to each well, and the plates incubated on a plate shaker at room temperature for 15 min. Cell lysates (10 μl) were transferred into wells of a 96-well white luminometer plate (Greiner Bio-One), and the luciferase assays were performed using a GloMax® 96 Microplate Luminometer (Promega) according to the manufacturer’s protocol.
96 well white luminometer plate
Manufactured by Greiner
The 96-well white luminometer plate is a laboratory equipment designed for luminescence-based assays. It provides a standardized and consistent platform for measuring light output from samples in a 96-well format. The plate is made of white polystyrene material to optimize light detection and minimize background interference.
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Lab products found in correlation
2 protocols using 96 well white luminometer plate
1
Dual-Luciferase Reporter Assay Protocol
2
Dual-Luciferase Assay for Retinoid Treatment
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Firefly and Renilla luciferases were measured sequentially using the Dual-Luciferase reporter assay system (Promega) according to the manufacturer's protocol. After 24 h of retinoid treatment, the medium was removed and the cells were washed with 250 μl of PBS; 100 μl of passive lysis buffer was then added to each well, and the plates incubated on a plate shaker at room temperature for 15 min. Cell lysates (10 μl) were transferred into wells of a 96-well white luminometer plate (Greiner Bio-One), and the luciferase assays were performed using a GloMax® 96 Microplate Luminometer (Promega) according to the manufacturer's protocol.
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