Aps200 system

Brains chosen for histological analysis were harvested postmortem from rabbits and GP that succumbed to the infection, or at designated time point (controls and non-lethal infections). The brains were immediately placed in 50 ml tubes containing ∼30 ml of 3.7% formaldehyde in PBS for fixation. After fixation, brains were cross-sectioned into 4–5 mm thick slices, each placed in a separate histological cassette, and the slices were paraffinized overnight in a Leica APS200 system (Leica Biosystems, Wetzler, Germany). The tissue slices were then embedded in paraffin blocks and consequently slides were prepared by mounting 5 µm thick sections prepared using a rotary microtome (Leica Biosystems, Wetzler, Germany).

Brains chosen for histological analysis were harvested from rabbits that succumbed to the infection, or at designated time points (controls). The brains were immediately placed in 50ml tubes containing ~30 ml of 3.7% formaldehyde in PBS for fixation. After fixation, brains were cross-sectioned into 4–5 mm thick slices, each placed in a separate histological cassette, and the slices were paraffinized overnight in a Leica APS200 system (Leica Biosystems, Wetzler, Germany). The tissue slices were then embedded in paraffin blocks. Consequently slides were prepared by mounting 5μm thick sections prepared using a rotary microtome (Leica Biosystems, Wetzler, Germany)

Immediately following postmortem harvest of selected brains, they were fixed in 50 mL tubes containing ~30 mL of 3.7% formaldehyde (in PBS). After fixation, 4–5 mm thick coronal brain sections were placed in separate histological cassettes. The cassettes were then paraffinized overnight using a Leica APS200 system (Leica Biosystems, Wetzler, Germany). The paraffinized tissue slices were embedded in paraffin blocks, and slides were prepared by mounting 5 μm thick sections cut on a rotary microtome (Leica Biosystems, Wetzler, Germany).