Fluorescence spectra were recorded on a Shimadzu RF-5301PC fluorometer. Fluorescence decays were collected on a HORIBA DeltaFlex modular fluorescence lifetime system with an excitation pulse at 520 nm. The absolute photoluminescence quantum yield (PLQY) was obtained by a time-correlated single photon counting spectrometer in combination with a FLS980 Edinburgh fluorescence spectrometer. UV-vis spectra measurement was performed on a Shimadzu UV-1800 spectrophotometer. Transmission electron microscopy (TEM) images were obtained by a FEI-TECNAI G2 microscope operating at 200 kV. Dynamic light scattering and zeta-potential results were accessed on a Brookhaven ZetaPlus apparatus. The energy electron spectroscopy (EDS) pattern was recorded on the AMETEK SEM-associated EDAX GENSIS energy dispersive spectroscopy. Fourier transform infrared (FTIR) spectra were collected with a Nicolet is 50 FTIR spectrometer scanning over the frequency range of 4000 to 400 cm−1 The fluorescence images were obtained with a reflected fluorescence microscope system with a digital camera DP80 from Olympus. The absorbance for MTT assay was recorded by a Molecular Devices SpectraMax Reader.
Zetaplus apparatus
Manufactured by Brookhaven Instruments
The ZetaPlus apparatus is a versatile instrument designed for the measurement of zeta potential and particle size. It utilizes the principle of electrophoretic light scattering to determine the zeta potential of dispersed samples. The instrument can also measure the hydrodynamic diameter of particles in suspension through dynamic light scattering.
Automatically generated - may contain errors
Lab products found in correlation
Rf 5301pc fluorometer, by Shimadzu (2 mentions)
Uv 1800 spectrophotometer, by Shimadzu (2 mentions)
Deltaflex, by Horiba (1 mentions)
Hemoglobin, by Sangon (1 mentions)
Guanidine hydrochloride, by Merck Group (1 mentions)
Ovalbumin (ova), by Merck Group (1 mentions)
Mos 500 circular dichroism spectrometer, by Bio-Logic (1 mentions)
Tecnai g2 twin microscope, by Thermo Fisher Scientific (1 mentions)
Glutathione (gsh), by Sangon (1 mentions)
Hydrogen tetrachloroaurate 3, by Merck Group (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
Human serum albumin (hsa), by Merck Group (1 mentions)
Rnase a, by Sangon (1 mentions)
Bca kit, by Sangon (1 mentions)
Lysozyme, by Sangon (1 mentions)
Trypsin, by Sangon (1 mentions)
Horseradish peroxidase, by Sangon (1 mentions)
Jem 2010 electron microscope, by JEOL (1 mentions)
Jem 3010 electron microscope, by JEOL (1 mentions)
Uv 1800 uv vis spectrophotometer, by Shimadzu (1 mentions)
4 protocols using zetaplus apparatus
1
Characterization of Nanomaterial Properties
2
Zeta-potential Analysis of Samples
3
Spectroscopic Characterization of Gold Nanoparticles
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Hydrogen
tetrachloroaurate(III) (≥99.9%), HSA, BSA, ovalbumin, lipase
and guanidine hydrochloride were purchased from Sigma. GSH, hemoglobin,
horseradish peroxidase, lysozyme, RNase A, carbonic anhydrase, immunoglobulin
G, trypsin, and BCA kit were purchased from Sangon Biotech. Phosphate
buffers (20 mM) with pH 4.0, 5.0, 6.0, 7.0, 8.0, and 9.0 were prepared
by mixing solutions of Na2HPO4 (20 mM) and NaH2PO4 (20 mM). The buffer pH was tuned by HCl/NaOH
solution.
Luminescence study was performed on a Shimadzu RF-5301PC
fluorometer. Time-resolved luminescence spectra were measured on an
Edinburgh FS 920 fluorometer. UV–vis spectra measurement was
performed on a Shimadzu UV-1800 spectrophotometer. TEM micrographs
were obtained by a FEI Tecnai G2-Twin microscope. XPS were obtained
using an ESCALAB-MKII spectrometer. CD spectra were carried out on
a Bio-Logic MOS 500 circular dichroism spectrometer. Secondary structural
contents were calculated by using the Dicro 2000 program. DLS and
ζ-potential measurement were performed on a Brookhaven ZetaPlus
apparatus.
tetrachloroaurate(III) (≥99.9%), HSA, BSA, ovalbumin, lipase
and guanidine hydrochloride were purchased from Sigma. GSH, hemoglobin,
horseradish peroxidase, lysozyme, RNase A, carbonic anhydrase, immunoglobulin
G, trypsin, and BCA kit were purchased from Sangon Biotech. Phosphate
buffers (20 mM) with pH 4.0, 5.0, 6.0, 7.0, 8.0, and 9.0 were prepared
by mixing solutions of Na2HPO4 (20 mM) and NaH2PO4 (20 mM). The buffer pH was tuned by HCl/NaOH
solution.
Luminescence study was performed on a Shimadzu RF-5301PC
fluorometer. Time-resolved luminescence spectra were measured on an
Edinburgh FS 920 fluorometer. UV–vis spectra measurement was
performed on a Shimadzu UV-1800 spectrophotometer. TEM micrographs
were obtained by a FEI Tecnai G2-Twin microscope. XPS were obtained
using an ESCALAB-MKII spectrometer. CD spectra were carried out on
a Bio-Logic MOS 500 circular dichroism spectrometer. Secondary structural
contents were calculated by using the Dicro 2000 program. DLS and
ζ-potential measurement were performed on a Brookhaven ZetaPlus
apparatus.
4
Characterization of Nanoparticle Dispersions
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UV-Vis spectra were recorded on a Shimadzu UV-1800 UV-Vis spectrophotometer. Transmission electron microscopy (TEM) observations were carried out with a JEOL JEM-2010 electron microscope with an acceleration voltage of 100 kV. High-resolution TEM (HRTEM) images were obtained by a JEOL JEM-3010 electron microscope operated with an acceleration voltage of 300 kV. The dispersions were dropped onto carbon-coated copper grids and then dried in air before the TEM observations. Temporal evolutions of the UV-Vis spectra were acquired by an Ocean Optics HR4000CG-UV-NIR high-resolution spectrophotometer. Zeta-potential was measured on a Brookhaven ZetaPlus apparatus. The pH of samples was adjusted from 2.5 to 8.0 by using Britton–Robinson buffer (mixture of 2 mM acetic acid, phosphate acid and boric acid with different amount of NaOH). Power XRD patterns were recorded on a Rigaku D-Max 2550 diffractometer equipped with a graphite monochromator using Cu Kα radiation (λ = 1.54 Å) at a scanning speed of 5° min−1. The samples for XRD measurements were dried frozenly in vacuo. Raman spectra were obtained by using a Horiba LabRAM UR evolution instrument with 785 nm laser (50 mW) and a holder for cuvette-based liquid measurements. The accumulation time was 60 s for all the data presented.
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