Anti human ttr

Manufactured by Agilent Technologies

Anti-human TTR is a laboratory reagent used for the detection and quantification of transthyretin (TTR) in human samples. TTR is a transport protein primarily produced in the liver and plays a role in the transport of thyroid hormones and retinol. The Anti-human TTR reagent can be used in various analytical techniques to measure TTR levels in biological samples.

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Lab products found in correlation

Dg100, by R&D Systems (1 mentions) Dgh00, by R&D Systems (1 mentions) Somascan assay platform, by SomaLogic (1 mentions) Enhanced chemi luminescence (ecl), by GE Healthcare (1 mentions) Vectastain abc ap kit, by Vector Laboratories (1 mentions)

Close spelling variants of this product

Rabbit anti-human TTR Polyclonal rabbit anti-human TTR antibody Rabbit anti-human TTR antibody Anti-TM

4 protocols using anti human ttr

Plasma Proteome Profiling for Bariatric Surgery

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Plasma proteome profiling was performed using the high-throughput DNA aptamer-based SOMAscan assay platform (SomaLogic, Inc.)^{78 (link)}. The abundance of 1129 proteins (enriched for extracellular proteins) was quantified as relative fluorescent units (RFU), normalized, calibrated, and log₂-transformed. Samples were available at baseline, 3, 12, 24, and 36 month time points for 19, 19, 19, 15, and 14 participants in the RYGB arm, and for 19, 19, 16, 10, and 9 participants in the DWM arm (Supplementary Data 1).
Selected proteomic data were validated by ELISA in a subset of fasting plasma samples, including IGFBP2 (22-BP2HU-E01, ALPCO, NH), CNDP1 (F34010, LifeSpan Biosciences, WA), growth hormone (DGH00, R&D Systems, MN), and total IGF-1 (DG100, R&D Systems, MN). RBP4 and TTR were assayed by quantitative western blotting using polyclonal anti-human RBP4 (Dako) and anti-human TTR (Dako) with standard curves of purified human RBP4 or TTR (Sigma) on each blot^{79 (link)}. Changes per individual over time were tested for positive correlation to corresponding SOMAscan changes with a one-sided test of Pearson correlation.

Dreyfuss J.M., Yuchi Y., Dong X., Efthymiou V., Pan H., Simonson D.C., Vernon A., Halperin F., Aryal P., Konkar A., Sebastian Y., Higgs B.W., Grimsby J., Rondinone C.M., Kasif S., Kahn B.B., Foster K., Seeley R., Goldfine A., Djordjilović V, & Patti M.E. (2021). High-throughput mediation analysis of human proteome and metabolome identifies mediators of post-bariatric surgical diabetes control. Nature Communications, 12, 6951.

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Semi-Denaturing Detection of TTR Conformers

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Twenty microliter CSF were subjected to electrophoresis in a 15% acrylamide gel without SDS. Samples were in sample buffer without SDS or reducing agent and loaded into the gel without denaturation at high temperature. TGS electrophoresis buffer was used to perform a semi-denaturing electrophoresis assay. Proteins were electroblotted onto PVDF membrane in a Semi-dry iBlot system (Invitrogen). After blocking, immunodetection was performed using anti-human TTR (DAKO) diluted (1/200) with 2.5% skimmed milk for 1 h. After washing with PBST, followed by incubation with sheep anti-rabbit immunoglobulins–HRP conjugated (Pierce; 1:5000 dilution), TTR was visualized using the enhanced chemiluminescence method (ECL, GE Healthcare). Densitometry and quantitative analysis of images were performed using Image J (NIH) software. Total conformers % was calculated by dividing the densitometry levels for the conformer fraction by the total TTR immunoreactivity (conformer, dimer and monomer).

Salazar I.L., Lourenço A.S., Manadas B., Baldeiras I., Ferreira C., Teixeira A.C., Mendes V.M., Novo A.M., Machado R., Batista S., Macário M.D., Grãos M., Sousa L., Saraiva M.J., Pais A.A, & Duarte C.B. (2022). Posttranslational modifications of proteins are key features in the identification of CSF biomarkers of multiple sclerosis. Journal of Neuroinflammation, 19, 44.

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Immunostaining of Atrial Proteins

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Adjacent sections of atrium were immunostained for A‐11 and either ANP or TTR. For ANP immunostaining, the same protocol described here for A‐11 was used with a rabbit polyclonal antibody directed against α‐ANP (1‐28; 1:200, Phoenix Pharmaceuticals, Inc) along with MF‐20. For TTR, a previously published protocol was used with modifications using a rabbit polyclonal anti–human‐TTR (1:500; DakoCytomation).^{24 (link)} For both proteins, a positive control preparation was generated by transfecting HEK or COS M6 cells with Myc‐DDK–tagged human TTR (OriGene Technologies, NM_000371) or human natriuretic peptide precursor A (NPPA NM_006172.1), respectively. Western blot–positive cells were centrifuged and embedded into paraffin.

Sidorova T.N., Mace L.C., Wells K.S., Yermalitskaya L.V., Su P., Shyr Y., Atkinson J.B., Fogo A.B., Prinsen J.K., Byrne J.G., Petracek M.R., Greelish J.P., Hoff S.J., Ball S.K., Glabe C.G., Brown N.J., Barnett J.V, & Murray K.T. (2014). Hypertension Is Associated With Preamyloid Oligomers in Human Atrium: A Missing Link in Atrial Pathophysiology?. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 3(6), e001384.

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Amyloid Detection in Femoral Osteochondral Slabs

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Each femoral osteochondral slab was fixed in 10% zinc-buffered formalin for 2 days, decalcified in a formic acid decalcifier ‘To Be Decalcified’ (TBD, Thermo Scientific) for 7 days, followed by paraffin embedding. Serial sections (9 µm each) were cut and stained with Congo red (Amyloid Special Stain Kit, Leica). The sections were examined using polarizing light microscopy that reveals apple green birefringence of amyloid (8 (link)).
For immunohistochemistry staining of human TTR, sections (4 µm each) were blocked with 10% goat serum for 30 minutes at room temperature. Anti-human TTR (1:400 dilution) from Dako (A0002) was applied with 0.1% Tween 20 and incubated overnight at 4°C. After washing with PBS, the sections were incubated with biotinylated goat anti-rabbit secondary antibody for 30 minutes at room temperature, and then incubated using the Vectastain ABC-AP kit (Vector Laboratories) for 30 minutes. Slides were washed, and sections were incubated with 3,3-diaminobenzidine tetrahydrochloride (DAB) substrate for 5–10 minutes. Specificity controls were obtained by replacing the primary antibody with nonimmune rabbit IgG (1 µg/mL). Staining was absent under these conditions.

Akasaki Y., Reixach N., Matsuzaki T., Alvarez-Garcia O., Olmer M., Iwamoto Y., Buxbaum J.N, & Lotz M.K. (2015). Transthyretin deposition in articular cartilage: a novel mechanism in the pathogenesis of osteoarthritis. Arthritis & rheumatology (Hoboken, N.J.), 67(8), 2097-2107.

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