Animals were anesthetized using ketamine hydrochloride (100 mg/kg, i.m.) and diazepam (5 mg/kg, i.m.) in combination and mounted on a wax tray. The ECG signals were recorded using platinum hook electrodes connected to bio amplifier (ML-136) and single channel PowerLab (ML-826) (AD Instruments, Australia). The ECG signals were used to perform HRV analysis (Labchard PRO-8, AD Instruments, Australia) using the method described previously (Roy et al., 2018 (link)).
Ml826
Manufactured by ADInstruments
Sourced in Australia
The ML826 is a high-performance data acquisition module designed for use in scientific and research applications. It features multiple input channels, high-resolution analog-to-digital conversion, and advanced signal processing capabilities. The core function of the ML826 is to capture, condition, and digitize a variety of analog signals with high accuracy and precision.
Automatically generated - may contain errors
Lab products found in correlation
Fe221, by ADInstruments (3 mentions)
Mlt1199, by ADInstruments (3 mentions)
Powerlab data acquisition system, by ADInstruments (3 mentions)
Ml136, by ADInstruments (1 mentions)
Labchart pro software, by ADInstruments (1 mentions)
Labchart pro v8, by ADInstruments (1 mentions)
Nitroprusside, by Merck Group (1 mentions)
Labchart pro, by ADInstruments (1 mentions)
4 protocols using ml826
1
ECG Signal Recording and HRV Analysis
2
Femoral Artery Cannulation for BP Monitoring
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A 1.5–2 cm incision was made in the inner part of the left leg to expose the femoral artery, where a heparinized (20 IU/ml) cannula (0.6 mm outer diameter) was inserted and secured using 4.0 silk sutures. The cannula was connected to a calibrated pressure transducer (AD-Instruments, MLT1199) and coupled to a bridge amplifier and power supply (AD-Instruments, FE221 and ML826, respectively). BP measurements were obtained continuously during the acute studies and exported at 1,000 and 100 bits per second, respectively. A PowerLab data acquisition system and LabChart Pro software (both from AD Instruments, Colorado Springs, CO, United States) were used to digitalize and visualize the data.
3
Femoral Artery Pressure Monitoring
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The mean arterial pressure (MAP) was measured with a catheter in the femoral artery, and the drug delivery was administrated in the femoral vein. n = 6 rats were used for this study, 1–3 physiological tests per rat. A 1.0–1.5 cm incision in the medial aspect of the leg was made to expose both vessels, and a cannula (0.6 mm outer diameter) previously filled with heparinized saline (20 IU/mL) was inserted and secured to the muscle using 4.0 silk sutures. The arterial cannula was connected to a previously calibrated pressure transducer (AD-Instruments, MLT1199) coupled to a bridge amplifier and power supply modulus (AD-Instruments, FE221 and ML826, respectively) for continuously MAP evaluation. The venous cannula was coupled to an infusion system to administrate the vasoactive drug, nitroprusside (5 mg/mL, a bolus of 2.5 µg/g weight; 71778 Sigma Aldrich). Simultaneous nerve activity was recorded with the sutrodes placed on the cVN and SNVP-1 to 4. PowerLab data acquisition system (AD Instruments, Colorado Springs, CO) and LabChart Pro V8 (AD Instruments, Colorado Springs, CO) software were used to process and analyze the mean arterial pressure data.
4
Femoral Artery MAP Monitoring during Nitroprusside
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The mean arterial pressure (MAP) was measured with a catheter in the femoral artery, and the drug delivery was administrated in the femoral vein. n=6 rats were used for this study, 1 to 3 physiological tests per rat. A 1.0-1.5 cm incision in the medial aspect of the leg was made to expose both vessels, and a cannula (0.6mm outer diameter) previously filled with heparinized saline (20 IU/mL) was inserted and secured to the muscle using 4.0 silk sutures. The arterial cannula was connected to a previously calibrated pressure transducer (AD-Instruments, MLT1199) coupled to a bridge amplifier and power supply modulus (AD-Instruments, FE221 and ML826, respectively) for continuously MAP evaluation. The venous cannula was coupled to an infusion system to administrate the vasoactive drug, nitroprusside (5mg/mL, a bolus of 2.5 µg/g weight; 71778 Sigma Aldrich). Simultaneous nerve activity was recorded with the sutrodes placed on the cVN and SNVP-1 to 4. PowerLab data acquisition system (AD Instruments, Colorado Springs, CO) and LabChart Pro (AD Instruments, Colorado Springs, CO) softwares
were used to process and analyze the mean arterial pressure data.
were used to process and analyze the mean arterial pressure data.
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