Recombinant ProGRP isoform 1 (AA 1−125 + 8) was cloned from human cDNA (OriGene Technologies, Rockville, MD,USA), expressed in Escherichia coli (Promega, Madison, WI, USA) using pGEX-6P-3 constructs (GE Healthcare Little Chalfont, UK) and purified as described elsewhere46 (link). Solutions of ProGRP and the Internal Standard (IS) NLLGLIEA[K_13C615N2] (purity >95%, Sigma-Aldrich) were prepared as described elsewhere21 (link).
Nllgliea k 13c615n2
Manufactured by Merck Group
Sourced in Germany
NLLGLIEA[K_13C615N2] is a laboratory-grade product from Merck Group. It is a synthetic peptide with a specific amino acid sequence and isotopic labeling. The core function of this product is to serve as a research tool for various applications in analytical and biochemical studies. Further details on its intended use are not available.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nllgliea k 13c615n2
1
Recombinant ProGRP Isoform 1 Production
2
Optimized Peptide Quantification Workflow
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Reagents and Standards. BSA with purity above 96% (Sigma-Aldrich, Germany) was dissolved in 50 mM freshly prepared ammonium bicarbonate (ABC) buffer and subsequently diluted to a final concentration of 48 nM. The target peptide NLLGLIEA[K_ 13 C6 15 N2] was purchased as AQUA Peptide and diluted according to the Custom AQUA Peptides Storage and Handling Guidelines by Sigma-Aldrich to a final concentration of 10 nM. TPCK-treated lyophilized trypsin from bovine pancreas was sequencing grade (Sigma-Aldrich).
All the solutions were stored at -20 °C. The MIP sorbents and the corresponding NIPs were provided from University of Malmö (Sweden) and prepared as described elsewhere 42 . All other chemicals used were of analytical grade.
All the solutions were stored at -20 °C. The MIP sorbents and the corresponding NIPs were provided from University of Malmö (Sweden) and prepared as described elsewhere 42 . All other chemicals used were of analytical grade.
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