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Mouse f4 80 bm8

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The Mouse F4/80 (BM8) is a cell surface marker that is expressed on mature tissue macrophages. It is commonly used for the identification and isolation of mouse macrophages in research applications.

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Lab products found in correlation

Ab102480, by Abcam (1 mentions) β actin ac 15, by Merck Group (1 mentions) Cleaved caspase 3 d175, by Cell Signaling Technology (1 mentions) Facsaria, by BD (1 mentions) Collagenase d, by Roche (1 mentions)

2 protocols using mouse f4 80 bm8

1

Apoptosis Induction and Analysis Protocol

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The following antibodies were used for Western blotting, confocal microscopy, and flow cytometry analyses: mouse F4/80 (BM8, eBioscience, San Diego, CA, USA), β-actin (AC-15, Sigma-Aldrich), mouse CD74 (05-591, Millipore), human Annexin V (ab14196, Abcam), and cleaved caspase-3 (D175, Cell Signaling). For chemical induction of apoptosis, cells were treated with 10 μM camptothecin at 37°C for 16 h using an apoptosis inducer kit (ab102480; Abcam).
Kim O.H., Kim H., Kang J., Yang D., Kang Y.H., Lee D.H., Cheon G.J., Park S.C, & Oh B.C. (2017). Impaired phagocytosis of apoptotic cells causes accumulation of bone marrow-derived macrophages in aged mice. BMB Reports, 50(1), 43-48.
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2

Spleen Immune Cell Isolation

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Spleen was digested in collagenase D (Roche, Basel, Switzerland) during 30 min at 37 °C then crushed and filtered on 70 µm tamis before staining. Single-cell suspensions were stained with mouse F4.80 (BM8, eBioscience), mouse CD11c (HL3, BD), and mouse B220 (RA3-6B2) antibodies from BD Biosciences. The HO-1 Intracellular stainings were performed using the BD cytofix/cytoperm kit with anti-HO-1 antibody (clone HO-1-1, abcam) followed by anti-mouse IgG1 (clone A85-1) conjugated to FITC. We used IgG1 (Immunotech) as negative controls. Stained cells were acquired on a FACSAriaTM (Becton Dickinson, Franklin Lakes, NJ, USA) flow cytometer and analyzed using Flow Jo v10.0.7 software (FlowJo LLC, Ashland, OR, USA).
Pogu J., Tzima S., Kollias G., Anegon I., Blancou P, & Simon T. (2019). Genetic Restoration of Heme Oxygenase-1 Expression Protects from Type 1 Diabetes in NOD Mice. International Journal of Molecular Sciences, 20(7), 1676.
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