Mouse urine corticosterone levels were measured by radioimmunoassay kit (MP Biomedicals, LLC). Serum α-MSH was measured using the Alpha-MSH Elisa kit (DRG). Serum IGF-1 was measured by mouse/rat IGF-1 Elisa kit (ALPCO). All assays were performed according to instructions provided by the manufacturer.
Mouse rat igf 1 elisa kit
Manufactured by ALPCO
Sourced in France
The Mouse/Rat IGF-1 ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the accurate measurement of insulin-like growth factor 1 (IGF-1) in mouse and rat serum, plasma, and cell culture supernatants. The kit utilizes a specific antibody coated on a 96-well plate and a horseradish peroxidase (HRP)-conjugated detection antibody to quantify the target analyte.
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2 protocols using mouse rat igf 1 elisa kit
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Mouse Hormone Measurement Methods
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Hormone Measurement Techniques in Mice
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Whole-blood GH concentrations were determined by EIA as described previously (Steyn et al. 2011) , using antibodies listed in Supplementary Table 1. Values are reported in terms of rGH-RP2. The detection limit was 0.038 ng/mL and the intraassay and interassay coefficients of variation were 3.2% and < 8.75%, respectively. Values are expressed as GH plasma concentrations.
IGF1 concentrations in plasma were determined with a Mouse/Rat IGF-1 ELISA kit (80-INSMSU-E01, ALPCO, Eurobio, Les Ulis, France) and leptin levels with a Mouse Leptin ELISA kit (EZML-82K, Merck Millipore, Molsheim, France) following the manufacturers' instruction manuals. IGF1 and leptin were assayed serially in both 3-and 12-month-old mice. Prolactin concentrations in plasma were determined by ultrasensitive ELISA assay as described previously (Guillou et al. 2015) (link).
IGF1 concentrations in plasma were determined with a Mouse/Rat IGF-1 ELISA kit (80-INSMSU-E01, ALPCO, Eurobio, Les Ulis, France) and leptin levels with a Mouse Leptin ELISA kit (EZML-82K, Merck Millipore, Molsheim, France) following the manufacturers' instruction manuals. IGF1 and leptin were assayed serially in both 3-and 12-month-old mice. Prolactin concentrations in plasma were determined by ultrasensitive ELISA assay as described previously (Guillou et al. 2015) (link).
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