Ritonavir (purchased from Toronto Research Chemicals, North York, ON, Canada), nelfinavir (purchased from Tocris Bioscience, Minneapolis, MN, USA), and valspodar (purchased from Santa Cruz Biotechnology, Santa Cruz, CA, USA) were dissolved in dimethyl sulfoxide and stored at −20°C until use.
Nelfinavir
Manufactured by Bio-Techne
Sourced in Canada, United States
Nelfinavir is a laboratory reagent manufactured by Bio-Techne. It is a protease inhibitor commonly used in research applications.
Automatically generated - may contain errors
Lab products found in correlation
Ritonavir, by LGC (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Caki 2, by American Type Culture Collection (1 mentions)
Cycloheximide (chx), by Enzo Life Sciences (1 mentions)
Celltiter 96 aqueous kit, by Promega (1 mentions)
Panobinostat, by LC Laboratories (1 mentions)
2 protocols using nelfinavir
1
Dissolution and Storage of Drug Compounds
2
Renal Cancer Cell Line Sensitivity to Panobinostat and Nelfinavir
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cell lines. Renal cancer cell lines (769-P, 786-O, Caki-2) were obtained from the American Type Culture Collection (Rockville, MD, USA). Cells were grown in Roswell Park Memorial Institute medium or McCoy's 5A medium supplemented with 10% fetal bovine serum and 1.0% penicillin/streptomycin (Invitrogen, Carlsbad, CA, USA) at 37˚C in a fully humidified atmosphere of 5% CO 2 .
Reagents. Panobinostat purchased from LC Laboratories (Boston, MA, USA) and nelfinavir purchased from Tocris Bioscience (Bristol, UK) were dissolved in dimethyl sulfoxide. Cycloheximide purchased from Enzo Life Sciences (Farmingdale, NY, USA) was dissolved in distilled water. All stock solutions were kept frozen at -20˚C until use.
Cell viability assay. Cells (5×10 3 ) were plated in a 96-well culture plate 1 day before treatment and then cultured for 48 hours in medium containing 15-60 nM Panobinostat with/without 10-20 μM nelfinavir. Cell viability was determined by MTS assay (CellTiter 96 Aqueous kit; Promega, Madison, WI, USA) following the manufacturer's instructions.
Reagents. Panobinostat purchased from LC Laboratories (Boston, MA, USA) and nelfinavir purchased from Tocris Bioscience (Bristol, UK) were dissolved in dimethyl sulfoxide. Cycloheximide purchased from Enzo Life Sciences (Farmingdale, NY, USA) was dissolved in distilled water. All stock solutions were kept frozen at -20˚C until use.
Cell viability assay. Cells (5×10 3 ) were plated in a 96-well culture plate 1 day before treatment and then cultured for 48 hours in medium containing 15-60 nM Panobinostat with/without 10-20 μM nelfinavir. Cell viability was determined by MTS assay (CellTiter 96 Aqueous kit; Promega, Madison, WI, USA) following the manufacturer's instructions.
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