An Agilent 1200 Series ChemStation Preparative HPLC system equipped with a diode array and a SUPELCOÒ SUPELCOSIL TM LC-18 column (5 mm, 250 x 21.2 mm) was used for the preparative purification of serratiochelin A, aerobactin, and pyoverdine Ag1. HPLC was monitored at 254 nm for the purification of compounds, unless otherwise stated. For all metabolomic studies, LCMS-ESI was conducted on an Agilent 6224 LC/MS-TOF (high resolution) system equipped with a diode array and an Agilent ZORBAX SB-C18 (5 mm, 2.1 x 50 mm) column. For MS/MS, an Agilent 6460 Triple Quadrupole LC-MS was used.
6224 lc ms tof high resolution system
Manufactured by Agilent Technologies
The 6224 LC/MS-TOF (high resolution) system is a liquid chromatography-mass spectrometry (LC/MS) instrument that utilizes time-of-flight (TOF) technology to provide high-resolution mass analysis. The core function of this system is to separate, detect, and identify chemical compounds with high precision and accuracy.
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2 protocols using 6224 lc ms tof high resolution system
1
Preparative HPLC and LCMS-TOF Analysis
2
Preparative HPLC and LC-MS/MS for Natural Product Purification
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An Agilent 1200 Series ChemStation Preparative HPLC system equipped with a diode array and a SUPELCO ® SUPELCOSIL TM LC-18 column (5 µm, 250 x 21.2 mm) was used for the preparative purification of serratiochelin A, aerobactin, and pyoverdine Ag1. HPLC was monitored at 254 nm for the purification of compounds, unless otherwise stated. For all metabolomic studies, LCMS-ESI was conducted on an Agilent 6224 LC/MS-TOF (high resolution) system equipped with a diode array and an Agilent ZORBAX SB-C18 (5 µm, 2.1 x 50 mm) column. For MS/MS, an Agilent 6460 Triple Quadrupole LC-MS was used.
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