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Fluorescentpolystyrene beads

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Fluorescent polystyrene beads are microscopic spherical particles composed of polystyrene polymer and embedded with fluorescent dyes. These beads are designed to emit light when exposed to specific wavelengths of light, allowing for their use in various applications that require the visualization or detection of small particles.

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Lab products found in correlation

Dmi6000, by Leica (2 mentions) Metamorph 6, by Molecular Devices (2 mentions) Dmem 4.5g l glucosemedium, by Thermo Fisher Scientific (2 mentions)

2 protocols using fluorescentpolystyrene beads

1

Tracheal Ciliary Function Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tracheal explants were prepared from N=7 wt and N=7
gal3-/- mice according to Delmotte and Sanderson (2006),
with minor modifications. The trachea were removed and sliced into 0.5mm-wide
rings. The rings were washed three times in pre-warmed DMEM 4.5g/l glucose
medium (Life Technologies) complemented with 25mM HEPES (pH 7.4) and mounted in
a home made chamber, in the same medium. This experiment was carried out using
three tracheal explants per animal. Video acquisitions of cilium beating profile
were performed using a LEICA DMI6000, a high-resolution camera CoolSnap HQ2
(Photometrics, Tucson, AZ) and MetaMorph 6 software (Molecular Devices,
Sunnyvale, CA). It was performed on N=7 wt and N=7 gal3-/-mice, using three tracheal explants per animal.
To record ciliary flow, suspensions of 0.2μm fluorescent
polystyrene beads (Sigma) were applied on N=6 wt and N=6
gal3-/- tracheal rings. Automatic bead tracking and
movement calculations were performed using IMARIS 7.0 (Bitplane, Zurich).
Clare D.K., Magescas J., Piolot T., Dumoux M., Vesque C., Pichard E., Dang T., Duvauchelle B., Poirier F, & Delacour D. (2014). Basal foot MTOC organizes pillar MTs required for coordination of beating cilia. Nature communications, 5, 4888.
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2

Tracheal Ciliary Function Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tracheal explants were prepared from N=7 wt and N=7
gal3-/- mice according to Delmotte and Sanderson (2006),
with minor modifications. The trachea were removed and sliced into 0.5mm-wide
rings. The rings were washed three times in pre-warmed DMEM 4.5g/l glucose
medium (Life Technologies) complemented with 25mM HEPES (pH 7.4) and mounted in
a home made chamber, in the same medium. This experiment was carried out using
three tracheal explants per animal. Video acquisitions of cilium beating profile
were performed using a LEICA DMI6000, a high-resolution camera CoolSnap HQ2
(Photometrics, Tucson, AZ) and MetaMorph 6 software (Molecular Devices,
Sunnyvale, CA). It was performed on N=7 wt and N=7 gal3-/-mice, using three tracheal explants per animal.
To record ciliary flow, suspensions of 0.2μm fluorescent
polystyrene beads (Sigma) were applied on N=6 wt and N=6
gal3-/- tracheal rings. Automatic bead tracking and
movement calculations were performed using IMARIS 7.0 (Bitplane, Zurich).
Clare D.K., Magescas J., Piolot T., Dumoux M., Vesque C., Pichard E., Dang T., Duvauchelle B., Poirier F, & Delacour D. (2014). Basal foot MTOC organizes pillar MTs required for coordination of beating cilia. Nature communications, 5, 4888.
+ Open protocol
+ Expand

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