The prepared paraffin sections were sequentially placed in Xylene I for 5 min and Xylene II for 5 min for dewaxing. Following dewaxing, gradient hydration was performed using ethanol, specifically, 5 min in absolute ethanol and 2 min each in 95%, 85%, and 75% ethanol and distilled water. Subsequently, the sections were immersed in hematoxylin stain (Solarbio Science & Technology Co., Ltd., Beijing, China) for 6 min for staining. After staining, differentiation, and bluing were performed using a hydrochloric acid–alcohol differentiation solution and bluing solution (Solarbio Science & Technology Co., Ltd., Beijing, China), respectively. Following rinsing with tap water, the sections were then immersed in eosin stain (Solarbio Science & Technology Co., Ltd., Beijing, China) for 5 min for staining. Post-staining, the sections were soaked in tap water for 5 min and dehydrated by soaking twice in 95% ethanol, absolute ethanol, and xylene for 1 min each. Finally, the sections were mounted with neutral balsam and observed under a light microscope (Olympus Sales & Service Co., Ltd., Tokyo, Japan) for H.E. staining evaluation.
Eosin stain
Manufactured by Solarbio
Sourced in China
Eosin stain is a commonly used dye in histology and cytology laboratories. It is a pink-colored anionic dye that primarily stains basic structures, such as the cytoplasm, collagen, and red blood cells, in biological samples. The core function of Eosin stain is to provide a contrasting color to the samples, allowing for better visualization and differentiation of cellular structures during microscopic examination.
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Lab products found in correlation
2 protocols using eosin stain
1
Hematoxylin and Eosin Staining Protocol
2
Renal Histopathological Evaluation Protocol
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Para n wax was serially sliced to a thickness of 5 μm. The sections were conventionally dewaxed with xylene and hydrated by various stages of ethanol. The section was stained for 5 min in hematoxylin (Solarbio, Beijing, China), and then rinsed with tap water. The section was differentiated for 30s in hydrochloric acid ethanol and then soaked in tap water for 15 min. The section was placed in Eosin stain (Solarbio, Beijing, China) for 2 min after that. Lastly, the section was routinely dehydrated, transparent and sealed. Renal histopathological changes were observed under a ×400 optical microscope (Olympus Model BX51, Olympus, Japan). A ve-point quantitative scoring method [20] was used to blindly measure the pathological changes of renal tissues according to the degree of renal tubular necrosis, swelling of renal tubular epithelial cells, vacuolization and shedding: 0, <10%; 1,10-25%; 2, 25-50%; 3, 50-75%; and 4, 75-100%.
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