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Female c57bl 6j wild type mice

Manufactured by Janvier Labs

Female C57BL/6J wild type mice are laboratory animals commonly used in biomedical research. They are genetically unmodified and serve as a standard control group in various experiments.

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2 protocols using female c57bl 6j wild type mice

1

Murine VSV-GP-Env Immunization

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Female C57BL/6J wild type mice were purchased from Janvier (Le Genest-Saint-Isle, France) and maintained in the animal facilities of the Innsbruck Medical University. Mice (8 per group) were immunized intramuscularly with 1 × 107 TCID50 of VSV-GP-Env constructs diluted in PBS at weeks 0, 4, and 8. Control animals received immunizations with VSV-GP. EDTA-blood was collected prior to the first immunization and 4 weeks after each immunization. Plasma was prepared and stored at −80 °C.
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2

Genetic Characterization of CXCR6 Mice

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Female C57BL/6J wild-type mice were purchased from Janvier Labs. CXCR6gfp/gfp mice, are homozygous CXCR6-deficient mice, in which the coding region of the chemokine receptor CXCR6 has been substituted with the coding region of the Enhanced Green Fluorescent Protein (eGFP). These mice were obtained from Jackson Laboratory (cat# JAX: 005693) and bred in our animal facility. Male CXCR6gfp/gfp mice were crossed with female C57BL/6J mice to generate CXCR6gfp/+ mice. B6.SJL-PtprcaPepcb/BoyJ (CD45.1) were purchased from Charles River. CD3 knockout (KO) mice were obtained from B Malissen’s laboratory (CIML, Marseille, France) and bred in our animal facility.
Mice were used in experiments at 8–10 weeks of age. All mice were housed in INSERM U970-PARCC animal facility under specific pathogen-free conditions.
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