Murine IL‐6 cytokine was purchased from R&D Systems. Murine GM‐CSF cytokine GM‐CSF was purchased from Abcam. Anti‐Ly6G (1A8) was purchased from eBioscience. STAT3 inhibitor was purchased from BioVision. Ethanol was from Sigma–Aldrich. Anti‐phosphorylated STAT3 (p‐STAT3) was purchased from CST. APC‐CD11b, FITC‐Ly6G, and PE‐Cy7‐Ly6C antibodies were purchased from BioLegend. APC‐CD3, FITC‐CD4, and APC‐Cy7‐CD8 antibodies were purchased from BD Biosciences. Berberine hydrochloride was purchased from Sigma–Aldrich. Lieber‐DeCarli liquid diet was purchased from Bio‐Serv.
Anti ly6g 1a8
Manufactured by Thermo Fisher Scientific
Anti‐Ly6G (1A8) is a monoclonal antibody that targets the Ly6G antigen, which is expressed on the surface of neutrophils. This antibody can be used for the detection and identification of neutrophils in various experimental and research applications.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 710, by Zeiss (2 mentions)
Cd8 apc cy7, by BD (1 mentions)
Cd3 apc, by BD (1 mentions)
Ly6g fitc, by BioLegend (1 mentions)
Lieber decarli liquid diet, by Bio-Serv (1 mentions)
Ly6c pe cy7, by BioLegend (1 mentions)
Berberine hydrochloride, by Merck Group (1 mentions)
Cd4 fitc, by BD (1 mentions)
Cd11b apc, by BioLegend (1 mentions)
Anti cd11c n418, by Thermo Fisher Scientific (1 mentions)
Anti inos m 19, by Santa Cruz Biotechnology (1 mentions)
Anti cd8 53 6, by Thermo Fisher Scientific (1 mentions)
Anti cd11b m1 70, by Thermo Fisher Scientific (1 mentions)
Anti cd11b m1 70, by BD (1 mentions)
Anti cd4 rm4 5, by BD (1 mentions)
Anti tnf α mp6 xt22, by BD (1 mentions)
Anti nk1.1 pk136, by BD (1 mentions)
Fc block, by BD (1 mentions)
Anticollagen 5, by Abcam (1 mentions)
Anti rabbit alexafluor 633, by Thermo Fisher Scientific (1 mentions)
3 protocols using anti ly6g 1a8
1
Murine Cytokine-Induced Immune Response
2
Multiparameter Flow Cytometry Analysis
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Equivalent cell numbers were treated with Fc block (BD Biosciences) and surface stained with combinations of anti-MHC II (M5/114.15.2), anti-CD11c (N418), anti-CD11b (M1/70), and anti-Ly6c (HK1.4) antibodies (eBiosciences) (neutrophils) or anti-Ly6g (1A8), anti-MHC class II (AF6–120.1), anti-CD11c (N418; eBioscience), anti-Ly6c (ER-MP20; AbD Serotech), anti-CD11b (M1/70; BD Biosciences), anti-CD4 (RM4.5; BD Biosciences), anti-NK1.1 (PK136; BD Biosciences), αβT cell receptor (H57–597; BD), anti-CD8 (53–6.7; eBioscience), anti-iNOS (M-19; Santa Cruz Biotechnology), or anti-TNF-α (MP6-XT22; BD Biosciences) (tipDCs, CD4+ T cells, CD8+ T cells) as previously described [37 (link),66 (link)]. CD8+ T cells were stained with tetramers specific for the DbPB1-F262–70 epitope for 1 h at room temperature prior to surface staining with the monoclonal antibodies as previously described [66 (link)].
3
Immunohistochemistry of Murine Liver Tissue
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Livers were embedded in optimal cutting compound and frozen in liquid nitrogen. 12 µm tissues sections were cut and fixed in 4% paraformaldehyde or acetone. Tissues were blocked in serum of the same species as the secondary antibody before incubation in primary antibody for 2 hours at room temperature. Tissues were then incubated in fluorochrome-conjugated secondary antibodies for 1 hour at room temperature prior to counterstaining with DAPI. Stained tissues were visualized using a laser scanning confocal microscope (LSM710, Carl Zeiss) with 10X or 20X lens. The following antibodies were used for immunohistochemistry: anti-Ly6G (1A8) 1:100 (eBioscience), anti-CD146 1:100, anti-CD31 1:100, anti-collagen V 1:200 (ABCAM), anti-FGF2 1:200 (Millipore), anti-CD66b 1:100 (Acris), anti-rabbit Alexafluor-633, anti-mouse AF-546 and anti-rat AF488 (all Life Technologies at 1:250).
For the human FGF2 ELISA on mouse serum we used a commercially available ELISA kit (Biolegend No434311). Serum was diluted 2-fold before addition to the ELISA plate and the protocol was followed as per the manual.
For the human FGF2 ELISA on mouse serum we used a commercially available ELISA kit (Biolegend No434311). Serum was diluted 2-fold before addition to the ELISA plate and the protocol was followed as per the manual.
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