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Fuji dri chem 3500i

Manufactured by Sysmex
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Sourced in Germany

The Fuji Dri-Chem 3500i is an automated chemistry analyzer designed for in-vitro diagnostic testing. It utilizes a dry chemistry technology to perform a variety of clinical chemistry tests on small sample volumes. The device is capable of analyzing multiple parameters simultaneously and provides rapid, accurate, and reliable results.

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Lab products found in correlation

Poch 100iv diff, by Sysmex (1 mentions) Cellquest pro, by BD (1 mentions) Facscalibur, by BD (1 mentions)

5 protocols using fuji dri chem 3500i

1

Measuring Troponin I and Lactate Dehydrogenase in Sepsis

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Troponin I was measured in 1:10 diluted serum samples by a commercially available ELISA kit (USCN, Life science Inc., Wuhan, China). The procedure was followed to manufacturer’s instructions. Lactate dehydrogenase was measured using the clinical chemistry analyzer Fuji Dri-Chem 3500i (Sysmex, Leipzig, Germany) according to manufacturer’s instructions. For analysis, data were obtained from n = 4 at baseline and at least n ≥ 7 animals following polymicrobial sepsis from each stratum and time point (SMPD1+/+, SMPD1−/−, dSMPD1+/+, dSMPD1−/−).
Chung H.Y., Kollmey A.S., Schrepper A., Kohl M., Bläss M.F., Stehr S.N., Lupp A., Gräler M.H, & Claus R.A. (2017). Adjustment of Dysregulated Ceramide Metabolism in a Murine Model of Sepsis-Induced Cardiac Dysfunction. International Journal of Molecular Sciences, 18(4), 839.
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2

Blood Profiling for Organ Dysfunction

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Blood was withdrawn by puncturing the cheek vein and collected in Li-Heparin-anticoagulated blood tubes. White blood cells (WBC), red blood cells (RBC), haematocrit (HCT) and platelets were analysed by automated veterinary haematology (Poch-100iv-Diff; Sysmex, Leipzig, Germany). In order to monitor organ failure, plasma was obtained by centrifugation of blood samples. Plasma levels of lactate dehydrogenase (LDH), glutamic-oxaloacetic transaminase (GOT) and glutamic-pyruvic transaminase (GPT) were determined using the clinical chemistry analyser (Fuji Dri-Chem 3500i; Sysmex, Leipzig, Germany).
Markwart R., Condotta S.A., Requardt R.P., Borken F., Schubert K., Weigel C., Bauer M., Griffith T.S., Förster M., Brunkhorst F.M., Badovinac V.P, & Rubio I. (2014). Immunosuppression after Sepsis: Systemic Inflammation and Sepsis Induce a Loss of Naïve T-Cells but No Enduring Cell-Autonomous Defects in T-Cell Function. PLoS ONE, 9(12), e115094.
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3

Hepatobiliary Dysfunction Assessment in Sepsis

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Serum from each stratum (smpd1+/+, smpd1+/−, p-smpd1+/+, t-smpd1+/+) at baseline, 24 hours, day 3 and 28 following sepsis induction was used for analysis of clinically used laboratory parameters of hepatobiliary damage and dysfunction (total-bilirubin T-Bil and γ-glutamyl-transferase γ-GT) using the clinical chemistry analyzer Fuji Dri-Chem 3500i (Sysmex, Leipzig, Germany) according to manufacturer’s instructions.
Chung H.Y., Witt C.J., Jbeily N., Hurtado-Oliveros J., Giszas B., Lupp A., Gräler M.H., Bruns T., Stallmach A., Gonnert F.A, & Claus R.A. (2017). Acid Sphingomyelinase Inhibition Prevents Development of Sepsis Sequelae in the Murine Liver. Scientific Reports, 7, 12348.
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4

Biomarkers of Cell Damage in Sepsis

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During the course of the systemic inflammation, clinically approved markers of cell damage—aspartate aminotransferase (AST) and lactate dehydrogenase (LDH)—were analysed in serum of wild-type (n = 8) as well as SMPD1 heterozygous littermates (n = 8) at baseline (control), 24 h and 28 days following sepsis induction using the clinical chemistry analyser Fuji Dri-Chem 3500i (Sysmex, Leipzig, Germany) according to manufacturer’s instructions.
Chung H.Y., Witt C.J., Hurtado-Oliveros J., Wickel J., Gräler M.H., Lupp A, & Claus R.A. (2018). Acid Sphingomyelinase Inhibition Stabilizes Hepatic Ceramide Content and Improves Hepatic Biotransformation Capacity in a Murine Model of Polymicrobial Sepsis. International Journal of Molecular Sciences, 19(10), 3163.
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5

Cytokine and Biomarker Profiling in Patients

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The plasma cytokine concentrations of IL-6, IL-8 and IL-10 were quantified using a flow cytometry-based multiplex assay according to the manufacturers instructions (BD, Cat. No. 551811). Data were acquired by FACS Calibur (BD) and BD CellQuest Pro software. Data were analyzed using the FCAP Array v1.0.1 software. Automated clinical chemistry analyzer (Fuji Dri-Chem 3500i; Sysmex, Germany) was used for measurements of lactate dehydrogenase activity within the supernatant. Other laboratory parameters as CRP, PCT (normal value <0.05 μg/l), blood count and plasma creatinine levels were also measured at automated clinical chemistry analyses as part of the standard laboratory procedures at the Jena university hospital.
Otto G.P., Hurtado-Oliveros J., Chung H.Y., Knoll K., Neumann T., Müller H.J., Herbsleb M., Kohl M., Busch M., Sossdorf M, & Claus R.A. (2015). Plasma Neutrophil Gelatinase-Associated Lipocalin Is Primarily Related to Inflammation during Sepsis: A Translational Approach. PLoS ONE, 10(4), e0124429.
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