MK-2206 (AKT inhibitor, A10003) was purchased from Selleckchem. GSK2334470 (PDK1 inhibitor, No. 4143) was purchased from Tocris Bioscience. AZD8055 (mTOR inhibitor, 1009298-09-2) was purchased from LC Laboratories. U0126 (MEK inhibitor, 70970-5) was purchased from Cayman Chemical.
Azd8055
AZD8055 is a selective and potent inhibitor of the mammalian target of rapamycin (mTOR) kinase. It functions by binding to the mTOR catalytic site, thereby inhibiting both mTOR complexes, mTORC1 and mTORC2.
Lab products found in correlation
6 protocols using azd8055
Signaling Pathway Inhibitor Protocol
MK-2206 (AKT inhibitor, A10003) was purchased from Selleckchem. GSK2334470 (PDK1 inhibitor, No. 4143) was purchased from Tocris Bioscience. AZD8055 (mTOR inhibitor, 1009298-09-2) was purchased from LC Laboratories. U0126 (MEK inhibitor, 70970-5) was purchased from Cayman Chemical.
Pharmacological Inhibitors in Cancer Research
Small Molecule Inhibitor Preparation
Pharmacological Evaluation of Diverse Compounds
Brassinosteroid Signaling Pathway Assay
Autophagy Induction and Carbon Starvation Assay
To induce autophagy, the seedlings incubated in the liquid medium for 7 d were transferred to nitrogen-deficient medium (1 × MS micronutrient solution, 3 mM CaCl2, 1.5 mM MgSO4, 1.25 mM KH2PO4, 5 mM KCl and 1% sucrose, pH 5.7) and further incubated for indicated duration. For drug treatment, seedlings were treated with 0.5 µM AZD 8055 (LC Laboratories), 30 µM wortmannin (Sigma), and 0.5 µM Concanamycin A (Cayman).
Phenotypic analysis of the fixed carbon starvation response was performed as described (Chung et al., 2010 (link)). Briefly, after incubating seedlings on 1× MS-suc solid medium (1× MS salt including vitamins, 29 mM mannitol, and 0.25% phytagel) for 14 d, the seedlings were then deprived of light for 10 d. Seedlings resuming growth were counted after 18 d of recovery.
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