Dubelcco s phosphate buffered saline dpbs

Manufactured by Thermo Fisher Scientific

Sourced in Switzerland, United States

Dulbecco's Phosphate Buffered Saline (DPBS) is a widely used buffer solution that maintains the pH and osmolarity of cell culture media. It is a sterile, isotonic solution composed of inorganic salts and is commonly used to wash, suspend, and dilute cells in various cell-based applications.

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Lab products found in correlation

Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Lapatinib, by Selleck Chemicals (2 mentions) 6 thioguanine, by Merck Group (2 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions) Temozolomide, by Merck Group (2 mentions) Trametinib, by Selleck Chemicals (2 mentions) Nintedanib, by Selleck Chemicals (2 mentions) Dimethyl sulfoxide (dmso), by Avantor (2 mentions) Zstk474, by Selleck Chemicals (2 mentions) Dimethyl sulfoxide (dmso), by Selleck Chemicals (2 mentions) Steponeplus real time pcr system, by Thermo Fisher Scientific (2 mentions) Powerup sybr green master mix, by Thermo Fisher Scientific (2 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions) Dnase 1, by New England Biolabs (1 mentions) Trypsin, by Thermo Fisher Scientific (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Thermo Fisher Scientific (1 mentions) Pei 25k, by Merck Group (1 mentions) Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)

5 protocols using dubelcco s phosphate buffered saline dpbs

Characterization of C. turicensis Strains

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C. turicensis LMG 23827^T26 (link), a clinical isolate responsible for two fatal sepsis cases in neonates in Zurich in 2006 was used in the study. Strains C. turicensis LMG 23827^T_Nal^R as well as C. turicensis LMG 23827^T/pRZT3::dsRed were described previously27 (link)28 .
For selection purposes, during zebrafish embryo infection experiments, C. turicensis LMG 23827^T_ΔrpfF/pCCR9 as well as C. turicensis LMG 23827^T_ΔrpfR/pCCR9 were constructed by transformation of the strains with the vector using standard methods.
Strains were grown in Luria–Bertani (LB) broth over night at 37 °C with gentle shaking. Where appropriate, culture medium or agar was supplemented with nalidixic acid at 256 mg L⁻¹ (C. turicensis LMG 23827^T_Nal^R), chloramphenicol at 30 mg L⁻¹ (strains harbouring pDS132) or both (transconjugant strains) or tetracyclin at 50 mg L⁻¹ (strains harbouring pCCR9 or pRZT3::dsRed).
For microinjection experiments, the bacteria were harvested by centrifugation at 5000 × g for 10 min and washed once in 10 ml of Dubelcco’s phosphate buffered saline (DPBS, Life Technologies, Switzerland.) After a second centrifugation step, the cells were resuspended in DPBS, and appropriate dilutions were prepared in DPBS.

Suppiger A., Eshwar A.K., Stephan R., Kaever V., Eberl L, & Lehner A. (2016). The DSF type quorum sensing signalling system RpfF/R regulates diverse phenotypes in the opportunistic pathogen Cronobacter. Scientific Reports, 6, 18753.

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Glioblastoma Cell Viability Assay

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Temozolomide (cat no. T2577) and 6-thioguanine (cat no. A4882) were purchased from Sigma and resuspended in DMSO (VWR scientific) to a concentration of 100 mM. Lapatinib (cat no. S2111), nintedanib (cat no. S1010), trametinib (cat no. S2673), and zstk474 (cat no. S1072) were purchased from Selleck Chemicals at a concentration of 10 mM in DMSO. Genetically perturbed pools of glioblastoma cells were seeded in 96-well plates at 2.5 x10⁴ cells per well in 100 μL of DMEM containing 10% FBS, 1% P/S, and 1 μg/mL puromycin and allowed to attach for 24 h. Small molecules were diluted to 1000-fold the exposure concentration in DMSO, followed by a 10-fold dilution into Dubelcco’s Phosphate buffered saline (DPBS, Life Technologies) and 1 μL added of the appropriate drug and dose to wells of seeded cells and a final concentration of 0.1% v/v DMSO. For Temozolomide and 6-thioguanine exposure experiments, cells were exposed for 96 h. For Lapatinib, nintedanib, trametinib and zstk474 experiments, cells were exposed for 72 h.

McFaline-Figueroa J.L., Srivatsan S., Hill A.J., Gasperini M., Jackson D.L., Saunders L., Domcke S., Regalado S.G., Lazarchuck P., Alvarez S., Monnat RJ J.r., Shendure J, & Trapnell C. (2024). Multiplex single-cell chemical genomics reveals the kinase dependence of the response to targeted therapy. Cell Genomics, 4(2), 100487.

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Glioblastoma Drug Screening Protocol

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Temozolomide (cat no. T2577) and 6-thioguanine (cat no. A4882) were purchased from Sigma and resuspended in DMSO (VWR scientific) to a concentration of 100 mM. Lapatinib (cat no. S2111), nintedanib (cat no. S1010), trametinib (cat no. S2673), and zstk474 (cat no. S1072) were purchased from Selleck Chemicals at a concentration of 10 mM in DMSO. Genetically perturbed pools of glioblastoma cells were seeded in 96-well plates at 2.5 ×10⁴ cells per well in 100 μL of DMEM containing 10% FBS, 1% P/S, and 1 μg/mL puromycin and allowed to attach for 24 hours. Small molecules were diluted to 1000-fold the exposure concentration in DMSO, followed by a 10-fold dilution into Dubelcco’s Phosphate buffered saline (DPBS, Life Technologies) and 1 μL added of the appropriate drug and dose to wells of seeded cells and a final concentration of 0.1% v/v DMSO. For Temozolomide and 6-thioguanine exposure experiments, cells were exposed for 96 hours. For Lapatinib, nintedanib, trametinib and zstk474 experiments, cells were exposed for 72 hours.

McFaline-Figueroa J.L., Srivatsan S., Hill A.J., Gasperini M., Jackson D.L., Saunders L., Domcke S., Regalado S.G., Lazarchuck P., Alvarez S., Monnat RJ J.r., Shendure J, & Trapnell C. (2023). Multiplex single-cell chemical genomics reveals the kinase dependence of the response to targeted therapy. bioRxiv.

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Absolute Copy Number Quantification of AAV

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AAVs were digested by DNaseI (New England BioLabs, Ipswich, MA, USA) prior to real-time qPCR [23 (link)] analysis. Briefly, a total of 2 × 10⁹ GC/mL of AAV (based on the concentrations provided by the vendor) was digested in 1× New England Biolabs DNase reaction buffer (New England BioLabs, Ipswich, MA, USA) with 2 U of DNaseI in 20 µL. The digestion mixtures were incubated at 37 °C for 15 min, followed by heat inactivation of DNase I at 75 °C for 10 min. The absolute copy number of the AAV genome was determined using a standard curve, with a linearized plasmid as the qPCR standard. Primers were designed to recognize the GOI. Both the linearized plasmid DNA and AAV samples were diluted in Dubelcco’s phosphate-buffered saline (DPBS) (ThermoFisher Scientific, Waltham, MA, USA). Real-time PCR was performed in quadruplicate using the PowerUp SYBR Green Master mix (Thermo Fisher Scientific) in a StepOnePlus Real-Time PCR System (Thermo Fisher Scientific), following the manufacturer’s protocol.

Wu D., Zhao X., Jimenez D.A, & Piszczek G. (2023). Size Exclusion Chromatography–Mass Photometry: A New Method for Adeno-Associated Virus Product Characterization. Cells, 12(18), 2264.

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Synthesis and Characterization of PEG-b-PAMPImB Polymer

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Borane-tert-butylamine complex (BTBA), hydrogen tetrachloroaurate (HAuCl₄·3H₂O) and branched poly(ethylenimine) (PEI25k) were purchased from Sigma-Aldrich and used as received. Ferrous chloride tetrahydrate (FeCl₂·4H₂O, >99% purity) and ferric chloride hexahydrate (FeCl₃·6H₂O, >99% purity) were bought from Shanghai Chemical Reagent Co., Ltd. (Shanghai, China). PEG₁₁₂-b-PAMPImB₉₆ was synthesized by RAFT polymerization by using PEG-CTA (Mw 5000) as macromolecular chain transfer agent and AMPImB as monomer. The detailed synthesis and characterization of this polymer was previously described [45 (link)]. Dulbecco’s modified Eagle’s medium (DMEM), penicillin–streptomycin, trypsin, fetal bovine serum (FBS), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT), and Dubelcco’s phosphate buffered saline (DPBS) were purchased from Thermo Fisher Scientific. The reporter plasmid, enhanced green fluorescent protein gene (pEGFP-C1), was amplified in E. coli and purified by E.Z.N.A.^® Endo-free plasmid DNA maxi kit (Omega). Purified pEGFP-C1 was stored at −20 °C and thawed at RT for the transfection assays.

Li J., Zou S., Gao J., Liang J., Zhou H., Liang L, & Wu W. (2017). Block copolymer conjugated Au-coated Fe3O4 nanoparticles as vectors for enhancing colloidal stability and cellular uptake. Journal of Nanobiotechnology, 15, 56.

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