Human dna damage pcr array

The cDNA were reverse transcribed from RNA using the First Strand cDNA Synthesis Kit (SABiosciences, Frederick, MD). Comparison of the relative expression of the 84 DNA damage related genes was characterized by human DNA Damage PCR array (SABiosciences) and the RT² real-time SYBR Green/Rox PCR master mix (SABiosciences) on a 7500 real time PCR system (Applied Biosystems, Rockville, MD). The array includes genes involved in apoptosis, cell cycle and damaged DNA binding and repair (Table S1). Data was analyzed using the RT² Profiler PCR Array Data Analysis v3.5 software (Qiagen). The fold change in gene expression was calculated using the equation 2(−ΔΔC_T). In cases in which a gene was down-regulated (less than 1 fold change), the value was reported as the negative inverse.

The cDNA was reversed transcribed from RNA employing the First strand cDNA synthesis Kit (SABiosciences, Frederick, MD, USA). Comparison of relative expression of 84 DNA damage related genes was determined by use of a human DNA damage PCR array (SABiosciences) and the RT² real-time SYBR Green/Rox PCR master mix (SABiosciences) utilizing a 7500 real time PCR system (Applied Biosystems, Rockville, MD, USA). The array encompasses genes associated with apoptosis, cell cycle and damaged DNA binding, and with DNA repair (Table S1).

The human DNA damage PCR array (SABiosciences, Frederick, MD) profiles expression of 84 genes involved in apoptosis, cell cycle and damaged DNA binding and repair (S1 Table). cDNA was prepared from RNA using the First strand cDNA synthesis Kit (SABiosciences, Fredrick, MD). Comparison of the relative expression of 84 genes was characterized (RT² real-time SYBR Green/Rox PCR master mix, SABiosciences) in 96 well microtiter plates on a 7500 real time PCR system (Applied Biosystems, Rockville, MD). Data was analyzed using the RT² profiler PCR Array Data Analysis v3.5 software (Qiagen). The fold change in gene expression was calculated using the equation 2(-ΔΔC_T). If the fold change was greater than 1, the result was considered as an up-regulation. For down-regulated (less than 1-fold change) genes the value was reported as the negative inverse.