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2 protocols using n n tetra acetic acid

1

Mitochondrial Bioenergetics and Antioxidant Analysis

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Glutamic acid, succinic acid, adenosine-5′-diphosphate sodium salt (ADP), cytochrome c from bovine heart, malic acid, ethylene glycol-bis-(b-aminoethylether)-N,N,N′N′-tetra acetic acid (EGTA), KH2PO4, TrisHCl, ethylenediamine tetra-acetic acid (EDTA), amytal, carboxyatractyloside (CAT), guanosine triphosphate (GTP)quercetin, acetonitrile, 2,2-azinobis (ethyl-2,3-dihydrobenzothiazoline-6-sulphonic acid) diammonium salt (ABTS), potassium persulfate, iron(III) chloride hexahydrate (FeCl3·6H2O), 2,4,6-tripyridyl-s-triazine (TPTZ), hydrochloric acid, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) were obtained from “Sigma–Aldrich GmbH”, Steinheim, Germany. Sucrose, mannitol, HEPES, KCl, MgCl2, trifluoroacetic acid, hyperoside, isoquercitrin, rutin were obtained from “Carl Roth Gmbh”, Karlsruhe, Germany.
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2

Isolation and Reconstitution of Mitochondrial Complexes

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2,4-Dinitrophenol (DNP), chloroform, dimethyl sulfoxide (DMSO), 1,2-dioleoyl-sn-glycero-3-phosphotidylcholine (DOPC), 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE), cardiolipin (CL), sodium sulfate (Na2SO4), 2-(N-morpholino) ethanesulfonic acid (MES), tris(hydroxymethyl)-aminomethane (Tris), adenosine 5′-triphosphate (ATP), guanosine 5′-triphosphate (GTP), carboxyatractyloside (CATR), ethylenediaminetetraacetic acid (EDTA), ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA), N-lauroylsarcosine, Triton X-114, dithiothreitol (DTT), bovine serum albumin (BSA), sucrose, 3-(N-morpholino) propanesulfonic acid (MOPS), rotenone, and safranin O were purchased from Sigma-Aldrich (Vienna, Austria). Arachidonic acid (AA) was purchased from Larodan (Solna, Sweden), n-octylpolyoxyethylene was purchased from BACHEM (Bubendorf, Switzerland), and hydroxyapatite was purchased from Bio-Rad Laboratories (Hercules, CA, USA).
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