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Human ferritin elisa kit

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The Human Ferritin ELISA Kit is a quantitative assay designed to measure the concentration of ferritin in human serum or plasma samples. Ferritin is an iron-storage protein found in the body, and its levels can provide information about iron status and certain medical conditions.

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Lab products found in correlation

Caco 2, by American Type Culture Collection (1 mentions) Penicillin streptomycin mix, by Merck Group (1 mentions) Transwell insert, by Merck Group (1 mentions) Non essential amino acids (neaa), by Merck Group (1 mentions) L glutamine, by Merck Group (1 mentions) Lucifer yellow, by Merck Group (1 mentions) Foetal bovine serum (fbs), by Euroclone (1 mentions) Atcc ccl 86, by American Type Culture Collection (1 mentions) Atcc htb 37, by American Type Culture Collection (1 mentions) Celltiter 96 aqueous one solution cell proliferation assay mts, by Promega (1 mentions)

Close spelling variants of this product

Ferritin (41 citations) ELISAs (4 citations)

4 protocols using human ferritin elisa kit

1

Bioavailable Iron Determination in Crickets

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Bioavailable iron in the crickets was determined by use of a well-established Caco-2 cell assay from Sandberg (60 (link)). The human colonic cancer cell line, Caco-2, was purchased from the American Type Culture Collection. Cells were grown in tissue culture treated flasks and kept in an incubator at 37°C and 5% CO2 in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum, 1% minimum essential media, and 1% penicillin. Cricket digest volumes were adjusted so that each treatment received ~300 nM of soluble iron. Cells were incubated for 2 h at 37° C and 5% CO2 and supplemented with an additional 0.5 mL minimal media. Cells were then incubated for 22 h, after which they were washed with PBS, lysed, centrifuged for 5 min at 16,000 × g, and the supernatant was collected. A Human Ferritin ELISA Kit (Sigma-Aldrich, Saint Louis, MO) was used to measure bioavailable iron following manufacturer’s instructions. Final concentrations were normalized to total protein to account for variation in Caco-2 cell number.
Ventura M., Holland M.E., Smith M.B., Chaparro J.M., Prenni J., Patz J.A., Paskewitz S., Weir T.L, & Stull V.J. (2023). Suitability of maize crop residue fermented by Pleurotus ostreatus as feed for edible crickets: growth performance, micronutrient content, and iron bioavailability. Frontiers in Nutrition, 10, 1157811.
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2

Serum Ferritin Quantification by ELISA

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Human serum ferritin concentration was measured by Human Ferritin ELISA kit (Sigma-Aldrich, RAB0197, St. Louis, MO). Briefly, 100 μL standard ferritin solutions and diluted serum samples (10-fold) were added into pre-coated wells of microtiter plates. The plates were then incubated overnight in cold room (4°C), with gentle shaking. Then the plates were incubated with 100 μL detection antibody for 1 hour after extensive washing (4 times) at room temperature. Next, 100 μL HRP-Streptavidin solution was added and incubated 45 min at room temperature. Finally, 100 μL ELISA Colorimetric was added and incubated for 30min at room temperature. The absorbance at 450um was determined for each well by Cytation 5 multi-mode plate reader (BioTek, Winooski, VT). The experiment was performed by duplicate and averaged values were used for calculation. The mean standard readings against their concentrations were used to prepare the best smooth curve. Ferritin protein concentrations from serum samples were then extrapolated from this curve. Genotypes were blinded to the experimenter.
Ma S., Dubin A.E., Zhang Y., Mousavi S.A., Wang Y., Coombs A., Loud M., Andolfo I, & Patapoutian A. (2021). A role of PIEZO1 in iron metabolism in mice and humans. Cell, 184(4), 969-982.e13.
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3

Ferritin ELISA Quantification Protocol

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Ferritin levels were measured in cell-free supernatants using Human Ferritin ELISA kit (RAB0197; Sigma) according to manufacturer’s protocol. Absorbance at 450 nm gave the measure of ferritin levels.
Gowda P., Patrick S., Joshi S.D., Kumawat R.K, & Sen E. (2021). Glycyrrhizin prevents SARS-CoV-2 S1 and Orf3a induced high mobility group box 1 (HMGB1) release and inhibits viral replication. Cytokine, 142, 155496.
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4

Caco-2 and Raji Cell-based Assays

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Caco-2 human epithelial colorectal adenocarcinoma cells (ATCC® HTB-37™) and human Burkitt’s lymphoma derived Raji cells (ATCC® CCL-86™) were purchased from ATCC (Manassas, VA, USA). High glucose Dulbecco’s Modified Eagle Medium (DMEM), Hanks’ Balanced Salt Saline (HBSS), non-essential amino acids (NEAA), L-glutamine, Penicillin-Streptomycin mix, reagents for simulant digestive fluids preparation, Lucifer Yellow (LY), and Human Ferritin ELISA Kit were purchased from Sigma-Aldrich (St Louis, MO, USA). Foetal bovine serum (FBS) was purchased from Euroclone (Milan, Italy). Transwell® insert was purchased from Millipore (Burlington, MA, USA). CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) was purchased from Promega (Madison, WI, USA). External standard for ICP-MS external calibration (IQC-026) was purchased from Agilent (Santa Clara, CA, USA), while internal standard (rodium) was purchased from Romil (Cambridge, Great Britain).
Pastore P., Roverso M., Tedesco E., Micheletto M., Mantovan E., Zanella M, & Benetti F. (2020). Comparative Evaluation of Intestinal Absorption and Functional Value of Iron Dietary Supplements and Drug with Different Delivery Systems. Molecules, 25(24), 5989.
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