Hek293t

The human embryonic kidney cell line HEK-293T (ATCC CRL-3216) and CRC cell lines HCT116 (ATCC CCL-247), HT29 (ATCC HTB-38), SW480 (ATCC CCL-228), and SW620 (ATCC CCL-227) were purchased from American Type Culture Collection (ATCC; http://www.atcc.org/). HCT116/L-OHP cells with oxaliplatin resistance were purchased from MEIXUAN Biological Science & Technology (Shanghai, China). The CRC cell lines HCT8, Caco2, RKO and LOVO were kindly provided by Professor Wancai Yang (Key Laboratory of Precision Oncology of Shandong Higher Education, Institute of Precision Medicine, Jining Medical University). The normal colon epithelial cell line 8401 (also known as CCD 841 CoN, ATCC® CRL­ 1790™) was kindly provided by Professor Lunquan Sun (Xiangya Hospital, Central South University, Changsha, China). HCT116 cells were maintained in McCoy’s 5 A medium (Biological Industries, Kibbutz Belt HaEmek, Israel) supplemented with 10% fetal bovine serum (FBS; Biological Industries, Kibbutz Belt HaEmek, Israel). The oxaliplatin-resistant cell line HCT116/L-CHOP was maintained in RPMI 1640 medium supplemented with 10000 ng/ml oxaliplatin (Selleck, Selleck Chemicals, USA). HEK-293T, HT29, SW480, SW620, HCT8, Caco2, RKO and LOVO cells were maintained in RPMI 1640 (Biological Industries, Kibbutz Belt HaEmek, Israel) supplemented with 10% FBS. All cells were cultured at 37 °C in the presence of 5% CO₂.

Human ESCC cell lines (KYSE-150 and TE-1) and a human embryonic kidney cell line (HEK293T) were purchased from the Institute of Biochemistry and Cell Biology of the Chinese Academy of Sciences (Shanghai, China). Cells were maintained in RPMI-1640 medium (KYSE-150 and TE-1) or DMEM (HEK293T) supplemented with 10% FBS (Biological Industries, Beit HaEmek, Israel) and 1% penicillin/streptomycin (Gibco) in a humidified incubator at 37 °C containing 5% CO₂. All cells used in this study were authenticated by short tandem repeat (STR) DNA profiling and used for experiments within 15 generations from initial resuscitation. The routine detection confirmed that all cells were free from mycoplasma contamination. The SB203580 was employed for p38 MAPK signal pathway inhibitory (ApexBio, Texas, USA).

Human GC cell lines AGS cells were purchased from the American Type Culture Collection (ATCC). HLECs, HEK-293T, BGC-823, HGC-27, MGC-803, SGC-7901, MKN-74, and MKN-45 were obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). HEK-293T cells were cultured in DMEM (Biological Industries, Cromwell, CT, United States), AGS cells were cultured in F12K medium (Biological Industries, Cromwell, CT, United States), and the other cells were cultured in RPMI-1640 medium (Biological Industries, Cromwell, CT, United States). All cells were cultured with 10% fetal bovine serum (FBS; Biological Industries, Cromwell, CT, United States), 100 U/ml penicillin (Invitrogen), 100 μg/ml streptomycin (Invitrogen) and incubated in 5% CO₂ at 37°C.

HEK293T, SH-SY5Y, and HT22 cells were purchased from ATCC. HEK293T and HT22 cells were cultured in Dulbecco’s minimum Eagle’s medium (Biological Industries) containing 10% (v/v) fetal bovine serum (Biological Industries) and 1% (v/v) penicillin/streptomycin (Invitrogen, CA, USA), while SH-SY5Y cells were maintained in a minimum essential medium (Biological Industries) supplemented with 10% (v/v) fetal bovine serum (Biological Industries) and 1% (v/v) penicillin/streptomycin at 37°C in a humidified atmosphere containing 5% CO₂. For lentivirus transfection, HT22 cells were seeded in 12-well culture plates at a cell density of 1 × 10⁵/well 1 day before transfection. Then, lentiviral vectors were transfected into cells by using polybrene. After 72–96 h, the cells were harvested for further analyses. For PMA treatment, PMA (MedChemExpress) was dissolved in DMSO, and SH-SY5Y cells were seeded in 10-cm culture plates at a cell density of 3 × 10⁷/plate 1 day before the treatment. Then, PMA was added to the medium with a final concentration of 100 nM. After 2 h, the cells were harvested for ChIP assay.

Human colorectal cancer cell lines (HCT116, SW620, HT29, SW480, HCT15, T84, RKO), and hepatocellular carcinoma cell lines (SMMC7721, HepG2, Bel7402), HEK293T, and RAW264.7 were purchased from the Type Culture Collection of Chinese Academy of Sciences (TCCCAS), Shanghai, China during the period from 2016 to 2018. All cell lines were authenticated by short tandem repeat profiling by TCCCAS before being purchased. HCT116, SW620, HT29, SW480, HCT15, and RKO cell lines were cultured in RPMI-1640 medium (Biological Industries, Kibbutz Beit-Haemek, Israel). SMMC7721, HepG2, Bel7402, HEK293T, and RAW264.7 cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Biological Industries). T84 was cultured in DMEM/F12 (1:1) medium (Gibco). All media were supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS, Biological Industries) and 1% (v/v) penicillin-streptomycin (Gibco) in a 37 °C incubator containing 5% CO₂.