Anti foxa1 antibody

Manufactured by Abcam

Anti-FOXA1 antibody is a laboratory reagent used to detect and study the FOXA1 protein. FOXA1 is a transcription factor involved in various cellular processes. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to identify and analyze the FOXA1 protein in biological samples.

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Lab products found in correlation

Anti sod2 antibody, by Abcam (1 mentions) Cm1850, by Leica (1 mentions) Alexa fluor 594, by Thermo Fisher Scientific (1 mentions) Fv300, by Olympus (1 mentions) Pcr purification kit, by Qiagen (1 mentions) Ab23738, by Abcam (1 mentions) Hiseq 2500, by Illumina (1 mentions) Paraformaldehyde (pfa), by Merck Group (1 mentions)

Spelling variants of this product

FOXA1 (23 citations) Anti-FOXA1 (17 citations) FOXA1 antibody (2 citations) Rabbit anti-Foxa1 antibody (2 citations)

4 protocols using anti foxa1 antibody

Immunohistochemical Profiling of Breast Cancer

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A total of 82 human primary breast cancer tissues were collected at Affiliated Hospital of Jiangnan University from years 2008 to 2012 with informed consent (Supplementary Table S3), and this project was approved by the Clinical Research Ethics Committees of Affiliated Hospital of Jiangnan University.
IHC staining was performed on 4 μm sections from formalin fixed paraffin embedded breast cancer tissues using anti-FOXA1 antibody (1:100, Abcam) and anti-SOD2 antibody (1:200, Abcam). FOXA1 nuclear expression was scored based on the staining intensity and the positive percentage of tumor cells as previously reported with some modifications 11 (link). Detailed evaluation method is described in Supplementary Table S4. For SOD2, IHC staining was observed in the cytoplasm of tumor cells, and a scale of 0 to 3 was used to score relative expression intensity. Chi-square test was conducted to examine the significance of FOXA1 or SOD2 IHC status in differentiating tumors of different subtypes.

Dai X., Cheng H., Chen X., Li T., Zhang J., Jin G., Cai D, & Huang Z. (2019). FOXA1 is Prognostic of Triple Negative Breast Cancers by Transcriptionally Suppressing SOD2 and IL6. International Journal of Biological Sciences, 15(5), 1030-1041.

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Immunohistochemical Analysis of FOXA1 in Human Placenta

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Human placenta samples were cut into small pieces and put into 4% paraformaldehyde at 4℃overnight for fixation. After that, the samples were treated with 30% sucrose for 24 hours to dehydrate. The samples were then embedded in OCT and sliced into 12 μm sections by using cryostat (LEICA CM 1850). The sections were blocked with 10% BSA for 1 hour at room temperature, and then treated with the primary antibody anti-FOXA1 antibody (1:200; Abcam) and incubated overnight at 4°C. After washing with PBS for three times, the samples were incubated with the secondary antibody conjugated to Alexa Fluor 594 (Invitrogen) for 1 h at room temperature. The fluorescent images of the samples were obtained using a laser scanning confocal microscope (FV-300, Olympus, Japan).

Wang Y., Zhang Y., Wang H., Wang J., Zhang Y., Wang Y., Pan Z, & Luo S. (2014). Aberrantly Up-regulated miR-20a in Pre-eclampsic Placenta Compromised the Proliferative and Invasive Behaviors of Trophoblast Cells by Targeting Forkhead Box Protein A1. International Journal of Biological Sciences, 10(9), 973-982.

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FOXA1 Binding Assay for AGR2 Promoter

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ChIP was performed according to the manufacturer’s recommendations (Active Motif, Carlsbad, CA). Anti-FOXA1 antibody (Ab23738, Abcam) was used to IP the TF/chromatin complex. DNA was purified from the IPed TF/chromatin complex using a PCR purification kit (Qiagen). The purified products were analyzed by real-time PCR. The primers against AGR2 promoter were as follows: sense 5ʹ-GGAAGGTTCGTTTCTGAGTT and anti-sense 5ʹ- AGCTGTGCTGGCACACTCAGACAGCT-3ʹ.

Yuan Z., Ye M., Qie J, & Ye T. (2020). FOXA1 Promotes Cell Proliferation and Suppresses Apoptosis in HCC by Directly Regulating miR-212-3p/FOXA1/AGR2 Signaling Pathway. OncoTargets and therapy, 13, 5231-5240.

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FOXA1 ChIP-Seq in RT4 Cells

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For identification of FOXA1 bound elements by ChIP-Seq, RT4 cells were cultured as described above, and crosslinked with 1% paraformaldehyde (Sigma). Following extraction of DNA/protein complexes, samples were incubated with anti-FOXA1 antibody (Abcam; ab5089). Sequencing libraries were generated using Rubicon ThruPLEX DNA sequencing kit (Rubicon Genomics, Ann Arbor MI). Sequencing was performed on an Illumina HiSeq2500 (Illumina, San Diego CA). For ChIP-Seq data analysis, raw FASTQ files were aligned to hg19 reference genome using bowtie2 (version 2.2.6)39 (link). Sam files were converted to bam using samtools (version 1.3)40 (link) and peaks were called using HOMER (version 4.8)41 (link). The two replicates were analyzed using Diffbind (version 2.05)42 R package where correlation coefficient and common peaks were extracted for further downstream analysis. De novo motif analysis for known transcription factors (TF) were generated with HOMER based on positional weight matrices from TRANSFAC (version 8.3). False-discovery rate of 5% was used as a cut-off.

Warrick J.I., Walter V., Yamashita H., Chung E., Shuman L., Amponsa V.O., Zheng Z., Chan W., Whitcomb T.L., Yue F., Iyyanki T., Kawasawa Y.I., Kaag M., Guo W., Raman J.D., Park J.S, & DeGraff D.J. (2016). FOXA1, GATA3 and PPARɣ Cooperate to Drive Luminal Subtype in Bladder Cancer: A Molecular Analysis of Established Human Cell Lines. Scientific Reports, 6, 38531.

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