Log In Sign up
The largest database of trusted experimental protocols

Cd144 apc

Manufactured by Miltenyi Biotec
Visit Supplier

CD144-APC is a fluorescent-labeled antibody that binds to the CD144 surface antigen. CD144 is also known as VE-cadherin, which is a cell-cell adhesion molecule expressed on endothelial cells. The APC fluorochrome attached to the antibody allows for the detection and analysis of CD144-positive cells by flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

Facsaria cell sorter, by BD (2 mentions) Fcr blocking reagent, by Miltenyi Biotec (2 mentions) Fortessa analyzer, by BD (2 mentions) Cd31 pe, by Miltenyi Biotec (1 mentions) Cd133 pe, by Miltenyi Biotec (1 mentions) Cd31 apc, by Miltenyi Biotec (1 mentions)

Spelling variants of this product

CD144 (4 citations)

2 protocols using cd144 apc

1

Flow Cytometry Analysis of Endothelial Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were harvested and suspended in ice-cold PBS with 1% BSA and 2 mM EDTA. After incubation with FcR Blocking Reagent (Miltenyi Biotec), cells were stained by fluorescently conjugated antibodies and incubated for 10 min in the dark in the refrigerator (2 − 8 °C). Antibodies include CD31-PE, CD144-APC, CD146-PE CD248-APC, IgG-APC and IgG-PE from Miltenyi Biotec (antibody information and dilution used is listed in the supplementary table 1). DAPI staining was used for dead cell exclusion. The stained cells or GFP and mCherry-labeled cells were analyzed in a BD Fortessa analyzer. FACS sorting was performed using the BD FACSAria cell sorter. Data were analyzed using FlowJo v10.8 software (https://www.flowjo.com/). A representative gating strategy for sorting mCherry+ cells is included in the source data.
Muthukrishnan S.D., Kawaguchi R., Nair P., Prasad R., Qin Y., Johnson M., Wang Q., VanderVeer-Harris N., Pham A., Alvarado A.G., Condro M.C., Gao F., Gau R., Castro M.G., Lowenstein P.R., Deb A., Hinman J.D., Pajonk F., Burns T.C., Goldman S.A., Geschwind D.H, & Kornblum H.I. (2022). P300 promotes tumor recurrence by regulating radiation-induced conversion of glioma stem cells to vascular-like cells. Nature Communications, 13, 6202.
+ Open protocol
+ Expand
2

Flow Cytometric Immunophenotyping of Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were harvested and suspended in ice-cold PBS with 1% BSA and 2mM EDTA. After incubation with FcR Blocking Reagent (Miltenyi Biotec), cells were stained by fluorescently conjugated antibodies and incubated for 10 min in the dark in the refrigerator (2–8°C). Antibodies include CD31-APC, CD144-FITC, CD144-APC, CD133-PE, IgG-APC, IgG-FITC, and IgG-PE from Miltenyi Biotec. The stained cells or GFP-labeled cells were analyzed in a BD Fortessa analyzer. FACS sorting was performed using the BD FACSAria cell sorter. Data were analyzed using FlowJo software.
Hu B., Wang Q., Wang Y.A., Hua S., Sauvé C.E., Ong D., Lan Z.D., Chang Q., Ho Y.W., Monasterio M.M., Lu X., Zhong Y., Zhang J., Deng P., Tan Z., Wang G., Liao W.T., Corley L.J., Yan H., Zhang J., You Y., Liu N., Cai L., Finocchiaro G., Phillips J.J., Berger M.S., Spring D.J., Hu J., Sulman E.P., Fuller G.N., Chin L., Verhaak R.G, & DePinho R.A. (2016). Epigenetic Activation of WNT5A Drives Glioblastoma Stem Cell Differentiation and Invasive Growth. Cell, 167(5), 1281-1295.e18.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!