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Pe cyanine7 anti mouse cd86 antibody

Manufactured by BioLegend
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The PE/Cyanine7 anti-mouse CD86 Antibody is a fluorescently labeled monoclonal antibody that binds to the mouse CD86 protein. CD86 is a co-stimulatory molecule that plays a role in T cell activation. The antibody is conjugated with a combination of the fluorescent dyes Phycoerythrin (PE) and Cyanine7, which allows for detection and analysis using flow cytometry.

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Lab products found in correlation

Fitc anti mouse cd45 antibody, by BioLegend (2 mentions) Fitc conjugated anti mouse cd4, by BioLegend (1 mentions) Pacific blue anti mouse human cd11b antibody, by BioLegend (1 mentions) Pe anti mouse cd206 mmr antibody, by BioLegend (1 mentions) Annexin 5 fitc pi apoptosis detection kit, by Yeasen (1 mentions) Ab109497, by Abcam (1 mentions) Ab90529, by Abcam (1 mentions) Apc anti mouse f4 80 antibody, by BioLegend (1 mentions) Ab209845, by Abcam (1 mentions) Ag 25b 006 c100, by Adipogen (1 mentions) Ag 20b 0042 c100, by Abcam (1 mentions) Pe anti mouse human cd11b antibody, by BioLegend (1 mentions)

2 protocols using pe cyanine7 anti mouse cd86 antibody

1

Radiolabeling and Immune Profiling

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All chemical reagents, purchased from Meryer Chemical (China), Zhengzhou Alfa (China), J&K (China), Energy Chemical (China), Xi’an Ruixi Biological Technology Co., Ltd (China) and Macrocyclics, Inc. Cell counting kit-8 (CCK-8) was purchased from Biyuntian Biotechnology Institute. Annexin V-FITC/PI Apoptosis Detection Kit was purchased from Yeasen Biotechnology (Shanghai) Co., Ltd. 89Zr was produced on an onsite cyclotron by the 89Y(p,n)89Zr reaction and purified to yield 89Zr(C2O4)2 solution. The cell Apoptosis 7-AAD Detection Kit was purchased from KeyGen Biotech Co., Ltd (Nanjing, China). FITC anti-mouse CD45 Antibody (#103107, 1:200), Pacific Blue™ anti-mouse/human CD11b Antibody (#101223, 1:100), APC/Cyanine7 anti-mouse F4/80 Antibody (#123117, 1:100), PE/Cyanine7 anti-mouse CD86 Antibody (#105013, 1:200), PE anti-mouse CD206 (MMR) Antibody (#141705, 1:100), PE-conjugated anti-mouse CD3 Antibody (#100205, 1:100), FITC-conjugated anti-mouse CD4 (#100406, 1:200), APC-conjugated anti-mouse CD8a Antibody (#100712, 1:200), Purified anti-HMGB1 Antibody (#651401, 1:100) were purchased from Biolegend. Mouse TNF-α, IL-6, IL-12p70 ELISA Kits and eFluor-450-conjugated anti-mouse Foxp3 antibody (#48-5773-82. 1:200) were obtained from Invitrogen. Alexa Fluor® 488 Anti-Calreticulin antibody [EPR3924] (ab196158, 1:100) was bought from Abcam.
Shi Y., Guo Z., Fu Q., Shen X., Zhang Z., Sun W., Wang J., Sun J., Zhang Z., Liu T., Gu Z, & Liu Z. (2023). Localized nuclear reaction breaks boron drug capsules loaded with immune adjuvants for cancer immunotherapy. Nature Communications, 14, 1884.
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2

Immunodetection of Inflammasome Components

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Rabbit monoclonal Anti-ASC (AG-25B-006-C100, AdipoGen, San Diego, CA, USA), mouse monoclonal anti-NLRP3 (AG-20B-0014-C100), rabbit monoclonal anti-caspase-1 (p20) (AG-20B-0042-C100), Rabbit monoclonal anti-GSDMD (ab209845, ABCAM, Cambridge, UK), rabbit monoclonal anti-PBR (ab109497, ABCAM), mouse Antiβ-actin (SJ190a9b68548, Sigma-Aldrich, St. Louis, MO, USA), rabbit monoclonal anti-IL-1β (12242, CST, Danvers, MA, USA), rabbit monoclonal anti-Iba1 (PA5-21274, Invitrogen), mouse monoclonal anti-Iba1 (019-19741, WAKO, Osaka Japan), anti-cytochrome C antibody (ab90529, Abcam) FITC anti-mouse CD45 antibody (103108, BioLegend, San Diego, CA, USA), APC anti-mouse F4/80 antibody (123116, BioLegend), PE/Cyanine7 anti-mouse CD86 antibody (105014, BioLegend), PE anti-mouse/human CD11b antibody (101208, BioLegend).
Zhang X., Han J., Xu Y., Cai M., Gao F., Han J., Wang D., Fu Y., Chen H., He W, & Zhang J. (2022). TSPO Deficiency Exacerbates GSDMD-Mediated Macrophage Pyroptosis in Inflammatory Bowel Disease. Cells, 11(5), 856.
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