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Cell proliferation elisa 5 bromo 2 deoxyuridine brdu colorimetric kit

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The Cell Proliferation ELISA 5-bromo-2′-deoxyuridine (BrdU) colorimetric kit is a laboratory assay used to measure cell proliferation. The kit utilizes the principle of BrdU incorporation into the DNA of proliferating cells, which can then be detected colorimetrically.

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Lab products found in correlation

Synergy ht, by Agilent Technologies (1 mentions)

Close spelling variants of this product

5-bromo-2′-deoxyuridine (BrdU; 5-bromo-2′-deoxyuridine (5-BrdU) 5-bromo-2-deoxyuridine Cell Proliferation ELISA BrdU Cell Proliferation ELISA BrdU ELISA kit Cell Proliferation Kit BrdU ELISA 5-BrdU ELISAs

2 protocols using cell proliferation elisa 5 bromo 2 deoxyuridine brdu colorimetric kit

1

Cell Proliferation Assay using BrdU

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Cell proliferation in cells was measured using the Cell Proliferation ELISA 5-bromo-2′-deoxyuridine (BrdU) colorimetric kit (#11647229001, Sigma Aldrich, St. Louis, MO). Transfected cells (5 × 103/well) were seeded into a 96-well plate format, incubated at 37 °C for 24 h and labeled with BrdU reagent for 24 h. Absorbance readings were taken at 370 nm and 492 nm (reference) using a Biotek Synergy HT plate reader and Gen5 version 1.08 software (BioTrek, Winooski, VT). Experiments included experimental groups with six replicates that were repeated at least three times.
Jones D.Z., Schmidt M.L., Suman S., Hobbing K.R., Barve S.S., Gobejishvili L., Brock G., Klinge C.M., Rai S.N., Park J., Clark G.J., Agarwal R, & Kidd L.R. (2018). Micro-RNA-186-5p inhibition attenuates proliferation, anchorage independent growth and invasion in metastatic prostate cancer cells. BMC Cancer, 18, 421.
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2

Cell Proliferation Assay Using BrdU

Check if the same lab product or an alternative is used in the 5 most similar protocols
The Cell Proliferation ELISA 5-bromo-2′-deoxyuridine (BrdU) colorimetric kit (Sigma-Aldrich, St. Louis, MO) was used to measure cell proliferation. This assay is based on BrdU incorporation into DNA in place of thymidine in replicating cells. Briefly, cells were grown in 96-well plates and were labeled with BrdU for 4 hours. The labeling medium was removed and cells were fixed using FixDenat. The anti-BrdU-POD antibody was added which formed a complex with newly synthesized cellular DNA that contained BrdU. The reaction complex was detected via absorbance at 450 nm using a multi-well spectrophotometer (Thermo Labsystems MultiskanEX model #355, Thermo Fisher Scientific, Waltham, MA).
Stewart C., Estrada A., Kim P., Wang D., Wei Y., Gentile C, & Pagliassotti M. (2017). Regulation of IRE1α by the small molecule inhibitor 4μ8c in hepatoma cells. Endoplasmic reticulum stress in diseases, 4(1), 1-10.
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