Moflow flow cytometer

Manufactured by Beckman Coulter

The MoFlow flow cytometer is an analytical instrument designed for the measurement and analysis of cells and particles in suspension. It utilizes the principles of flow cytometry to provide quantitative and qualitative data on various cellular properties, such as size, granularity, and fluorescence. The MoFlow is capable of rapidly processing and analyzing multiple parameters of individual cells or particles within a sample.

Automatically generated - may contain errors

Lab products found in correlation

Mouse igg1, by Thermo Fisher Scientific (1 mentions) Rat igg2a, by Miltenyi Biotec (1 mentions) Mouse igg1, by Miltenyi Biotec (1 mentions) Influx flow cytometer, by BD (1 mentions) Sytox blue, by Thermo Fisher Scientific (1 mentions) Cyan adp flow cytometer, by Beckman Coulter (1 mentions)

2 protocols using moflow flow cytometer

Isolation and Characterization of Stromal Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Isolated stromal cells (up to 1 x 10⁷ cells/100 μl) were incubated with antibody combinations of allophycocyanin (APC)-conjugated CD49f (1:10, clone GoH3, rat IgG2a; Miltenyi Biotec) and Alexa Fluor 488-conjugated CD45 (1: 20; mouse IgG1; Life Technologies) or phycoerythrin (PE)-conjugated CD271 (1:10, mouse IgG1; Miltenyi Biotec) and APC-conjugated CD49f in 2% fetal bovine serum/PBS (FBS/PBS) for 30 min on ice in the dark. Cells were then washed and resuspended in 1 μM Sytox Blue to distinguish live and dead cells (Life Technologies) and fluorescence activated cell sorting (FACS) was undertaken on a MoFlow flow cytometer (Beckman Coulter) or an Influx flow cytometer (Becton Dickinson Biosciences).

Letouzey V., Tan K.S., Deane J.A., Ulrich D., Gurung S., Ong Y.R, & Gargett C.E. (2015). Isolation and Characterisation of Mesenchymal Stem/Stromal Cells in the Ovine Endometrium. PLoS ONE, 10(5), e0127531.

+ Open protocol

+ Expand

Induction of Regulatory T Cells from Naïve CD4+ T Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Splenocytes from NOD mice were stained with anti-CD4 and anti-CD25 antibodies, and then sorted using a MoFlow flow cytometer (Beckman.Coulter). CD4⁺CD25⁻ (5×10⁵ cells) were then cultured in the presence of anti-CD3 (2µg/mL) and TGF-β (3 ng/mL) or anti-CD3 alone (control) for 48 hrs. Subsequently, the cells were washed thoroughly to remove anti-CD3/TGF-β, labeled with CFSE and then co-cultured for 5 days with G-BMDCs or SpDCs at a ratio of 2:1 (1 × 10⁵ T cells: 5 × 10⁴ DCs). Cells were then stained for CD4, Foxp3 and analyzed for Treg proliferation using Cyan ADP flow cytometer (Beckman/Coulter).

Haddad C.S., Bhattacharya P., Alharshawi K., Marinelarena A., Kumar P., El-Sayed O., Elshabrawy H.A., Epstein A.L, & Prabhakar B.S. (2016). Age-dependent divergent effects of OX40L treatment on the development of diabetes in NOD mice. Autoimmunity, 49(5), 298-311.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!