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Pe anti human cd13

Manufactured by BioLegend
Sourced in United States

PE anti-human CD13 is a fluorescently labeled antibody that binds to the CD13 antigen expressed on the surface of human cells. CD13 is a marker for myeloid cells, including granulocytes, monocytes, and their precursors. This product can be used for the identification and enumeration of CD13-positive cells in flow cytometry applications.

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2 protocols using pe anti human cd13

1

Humanized Mouse Model for Hematopoietic Engraftment

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For humanization, mice received total body irradiation (TBI) to a dose of 2 Gy. Irradiations were performed between 8–10 AM on unanesthetized mice using a SmART Precision X-ray instrument (225 kVp; 10 mA; half value layer (HVL) 0.89 mm Cu) with a dose rate of 107.7 cGy/min. At 24 hours post-irradiation, animals were transplanted via tail vein with a dose of 50,000 CD34+ cells from mixed donors purchased from StemCell technologies (catalog number: 70008). At four weeks post-CD34+ transplant, human CD45+ chimerism was determined by FACS analysis of peripheral blood. The following antibody panel was used: APC anti-human CD45 (Biolegend, 3368512), Pacific Blue Anti-human CD3 (Biolegend, 300330), FITC anti-mouse CD45 (Biolegend, 103108), PE anti-human CD13 (Biolegend, 301704), 7-AAD (Biolegend, 420404), APC-CY7 anti-human CD19 (Biolegend, 302218).
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2

Immune Cell Phenotyping Protocol

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Blood samples were centrifuged and washed four times with PBS. The buffy coat was collected from the blood with a pipette. Virus infection and fixation followed the procedure described above. The cells were stained with DAPI, PerCP-Cy5.5 anti-human CD45 (304027; BioLegend, San Diego, CA, USA); and one of the following antibodies: PE anti-human CD2 (309207), PE anti-human CD13 (301703), PE anti-human CD14 (301805), PE anti-human CD15 (301905), PE anti-human CD19 (302207), PE anti-human CD45 (304008) (all from BioLegend), and PE anti-human CD203c (130092243; Miltenyi Biotec Inc., San Diego, CA, USA).
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