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Ki67 primary antibody

Manufactured by Proteintech
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The Ki67 primary antibody is a protein marker that is expressed during all active phases of the cell cycle (G1, S, G2, and mitosis), but is absent in resting cells (G0). This antibody is commonly used in immunohistochemistry and flow cytometry applications to assess cell proliferation.

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Lab products found in correlation

Pcna antibody, by Proteintech (1 mentions) Sf3a3, by Abcam (1 mentions)

Close spelling variants of this product

Ki67 antibody (5 citations) Anti-Ki67 primary antibody (3 citations)

2 protocols using ki67 primary antibody

1

Immunohistochemical Analysis of Cell Proliferation

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Formalin‐fixed and paraffin‐embedded tissue sections were incubated with Ki67 primary antibody (dilution 1:1000; Proteintech), PCNA antibody (dilution 1:300; Proteintech), and SF3A3 (dilution 1:200; Abcam) overnight at 4 °C. Then, the sections were incubated with HRP-conjugated anti-rabbit IgG secondary antibody (dilution 1:300; Yeasen) for 2 h at room temperature. The slides were evaluated by two independent observers.
Chen D., Zhou H., Cai Z., Cai K., Liu J., Wang W., Miao H., Li H., Li R., Li X., Chen Y., Wang H.Y, & Wen Z. (2022). CircSCAP interacts with SF3A3 to inhibit the malignance of non-small cell lung cancer by activating p53 signaling. Journal of Experimental & Clinical Cancer Research : CR, 41, 120.
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2

Quantifying Proliferation in HFSCs

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HFSCs were washed with PBS, fixed with 4% paraformaldehyde, and incubated with Ki67 primary antibody (1:250, Cat No. 27309-1-AP, Proteintech, Wuhan, China) overnight. Detection was performed with an antirabbit secondary antibody (1:20,000, Cat No.SA00009-2, Proteintech, China). Cell nuclei were stained with DAPI. Pictures were quantified with ImageJ software.
Li J., Zhao B., Yao S., Dai Y., Zhang X., Yang N., Bao Z., Cai J., Chen Y, & Wu X. (2023). Dermal PapillaCell-Derived Exosomes Regulate Hair Follicle Stem Cell Proliferation via LEF1. International Journal of Molecular Sciences, 24(4), 3961.
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