All chemical reagents including methanol, petroleum ether (PE), chloroform (CF), ethyl acetate (EtOAc), and n-butanol (n-BuOH) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Folin-Ciocalteu, pyrogallol, and other analytical grade chemicals were purchased from Aladdin (Shanghai, China). Chromatographic acetonitrile was purchased from Merck (Darmstadt, Germany). Gallic acid and other standards for HPLC were purchased from Yuanye Bio-Technology (Shanghai, China). Cellulase (3000 U/g) and pectinase (40,000 U/g) were purchased from Xiya reagent (Shandong, China). α-glucosidase from Saccharomyces cerevisiae (G5003), acarbose, 4-methylumbelliferyl α-D-glucopyranoside (4-MUG), acetylcholinesterase (AChE) from Electrophorus electricus (C3389), galanthamine (GAL), acetylthiocholine iodide (ATCI), 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB), tyrosinase from mushroom (T3824), kojic acid, 3,4-Dihydroxy-L-phenylalanine (L-dopa), cisplatin, and streptozocin (STZ) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
The MCF-7 and HepG2 cells were purchased from Conservation genetics CAS Kunming cell bank. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and other cell culture reagents were purchased from Gibco (Carlsbad, CA, USA). Cell counting kit-8 (CCK-8) reagent was purchased from Dojindo (Kumamoto, Japan).
The MCF-7 and HepG2 cells were purchased from Conservation genetics CAS Kunming cell bank. Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and other cell culture reagents were purchased from Gibco (Carlsbad, CA, USA). Cell counting kit-8 (CCK-8) reagent was purchased from Dojindo (Kumamoto, Japan).