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Calmodulin from bovine testes

Manufactured by Merck Group
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Calmodulin from bovine testes is a calcium-binding protein. It plays a key role in the regulation of a number of enzymes by modulating their activity in response to changes in intracellular calcium concentration.

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Lab products found in correlation

Adenosine 5 triphosphate atp disodium salt hydrate, by Merck Group (1 mentions) Hepes buffer, by Merck Group (1 mentions) Adenosine 3 5 cyclic monophosphate, by Merck Group (1 mentions) Mgcl2, by Merck Group (1 mentions) Cacl2, by Merck Group (1 mentions) Methanol, by Thermo Fisher Scientific (1 mentions) Direct q purification system, by Merck Group (1 mentions)

2 protocols using calmodulin from bovine testes

1

Superparamagnetic Bead-Based Anthrax Toxin Assay

Cited 1 time
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Superparamagnetic Dynabeads MyOne Tosyl-activated beads were from Life Technologies (Carlsbad, CA). Recombinant EF was produced as previously described [17 (link)]. Activated recombinant PA (PA63) was from List Biological Laboratories (Campbell, CA). Pertussis adenylyl cyclase toxin (CyaA) was from Sigma-Aldrich (St. Louis, MO). Adenosine 5′-triphosphate (ATP) disodium salt hydrate, adenosine-13C10,15N5 5′-triphosphate sodium salt solution–labeled ATP (L-ATP), adenosine 3′,5′ cyclic monophosphate, Hepes buffer, CaCl2, MgCl2, calmodulin from bovine testes, and all other chemicals and reagents were from Sigma-Aldrich, except where indicated. A Kingfisher 96 magnetic purification system (ThermoFisher Scientific Inc., Waltham, MA) was used for automated sample preparation.
Lins R.C., Boyer A.E., Kuklenyik Z., Woolfitt A.R., Goldstein J., Hoffmaster A.R., Gallegos-Candela M., Leysath C.E., Chen Z., Brumlow J.O., Quinn C.P., Bagarozzi DA J.r., Leppla S.H, & Barr J.R. (2019). Zeptomole per milliliter detection and quantification of edema factor in plasma by LC-MS/MS yields insights into toxemia and the progression of inhalation anthrax. Analytical and bioanalytical chemistry, 411(12), 2493-2509.
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2

Calmodulin Solubilization and Purification

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Calmodulin from bovine testes was purchased from Sigma-Aldrich (Dorset, UK). HPLC grade methanol and formic acid (HAc), were obtained from Fisher Scientific (Loughborough, UK). Water was purified by a Millipore Direct-Q purification system (Millipore, Nottingham, UK).
CaM (0.4 μM) was dissolved in a 75:25 water/methanol (v/v) solution with 0.3% (v/v) HAc.
Floris F., Chiron L., Lynch A.M., Barrow M.P., Delsuc M.A, & O’Connor P.B. (2018). Application of Tandem Two-Dimensional Mass Spectrometry for Top-Down Deep Sequencing of Calmodulin. Journal of the American Society for Mass Spectrometry, 29(8), 1700-1705.
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