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6 protocols using cupric sulfate

1

Multifunctional Biomaterial Synthesis

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Barium carbonate (99%), titanium dioxide (99%), calcium carbonate (99.9%), zirconium dioxide (99.99%), cupric sulfate (99%), and nano HA were from Sinopharm Chemical Reagent Co., Ltd. (China). Dopamine hydrochloride (DA-HCl), tri(hydroxymethyl) amino methane hydrochloride (Tris-HCl, 1 M), methylene blue (MB), vancomycin, Calcein, and ARS were from Sigma Chemical Co. (USA). SOSG was from Invitrogen (USA). The Bacterial Live/Dead Bac Light viability kit was from Thermo Fisher (USA). Calcein/PI Cell Viability/Cytotoxicity Assay Kit, CCK-8 kit, and ARS dye were purchased from Beyotime (China).
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2

Synthesis and Characterization of PEG-based Antimicrobial

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Epichlorohydrin (Dow Chemical, USA), trifluoride diethyl etherate (BF3–OEt2, Tianjin Lianxing Biotechnology Co. Ltd.), 3-bromo-1-propyne (Aladdin, Shanghai), sodium ascorbate (VcNa, Aladdin, Shanghai), trypticase soy agar medium (TSA, AOBOX, Hengshui), trypticase soy liquid medium (TSB, AOBOX, Hengshui), 1,4-butylene glycol (BDO, KESHI, Chengdu), 4,4′-diphenylmethane diisocyanate (MDI, J&K, Beijing), tetrahydrofuran (THF, Bioway, Shanghai), anhydrous magnesium sulfate (MgSO4, Tianjin ZhiYuan Reagent Co. Ltd.) N,N-diisopropylethylamine (DIEA, J&K, Beijing) can be used directly. All other chemical reagents such as sodium hydride (NaH), dimethyl sulfoxide (DMSO), sodium azide (NaN3), methanol, sodium hydroxide (NaOH), diethyl ether, cupric sulfate (CuSO4), phosphate buffer (PBS), polypropylene glycol (PPG), methylene chloride (DCM) and dimethylformamide (DMF) were purchased from Sinopharm. S. aureus and E. coli were purchased from Guangdong Institute of Microbiology. mPEG with number average molecular weights of 5000, 3350 and 1000 (mPEG-5000, mPEG-3350 and mPEG-1000, Aladdin) were used without further purification.
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3

Electrospinning of Polymeric Tubular Grafts

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Poly (ε-caprolactone) (PCL, MW 80,000) and poly (l-lactide-co-caprolactone) (PLCL, MW 80,000) were purchased from Daigang Biomaterial Co. Ltd. (Jinan, China). ethylenediamine tetraacetic acid disodium salt, ethylenediamine, sodium chloroacetate, and cupric sulfate were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). The circular metal rod (Ф 2 mm) was used as a collector for electrospinning tubular grafts. All other chemicals were analytical grade and used without further purification.
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4

Versatile Nanodiamond-based Analytical Platform

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Nano-diamond, FeCl3, H2SO4 and HNO3, 3,3′,5,5′-tetramethylbenzidine (TMB), 1,2-diaminobenzene (OPD), 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) (ABTS), L-γ-glutamyl-L-cysteinylglycine (GSH), thiourea were purchased from Macklin reagent Co.,Ltd. (Shanghai, China), Human serum albumin (HAS), Bovine serum albumin (BSA), ascorbic acid (AA), glycine (Gly), L-lysine (Lys), L-serine (Ser), D/L-cysteine(D-Cys, L-Cys), glucose, sodium chloride, calcium chloride, cupric sulfate, ferric chloride, potassium chloride, zinc sulfate, magnesium sulfate, tartaric acid, choline chloride were purchased from Sinopharm Chemical Reagent Co. Ltd. NaN3 was purchased fromTianjin Fuchen Chemical Reagent Factory. H2O2 was purchased from Xilong Scientific Co., Ltd. sodium chloride, calcium chloride, cupric sulfate, ferric chloride, potassium chloride, zinc sulfate, magnesium sulfate, tartaric acid, choline chloride was purchased fromSinopharm ChemicalReagent Co., Ltd. Sodium acetate buffer (0.1 M, pH = 4.0) were freshly prepared before use. All solutions prepared for purified water are derived from Wahaha purified water (China).
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5

Investigating Heavy Metal Toxicity in Rats

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Heavy metal compounds, lead acetate ((CH3COO)2Pb·3H2O, AR ≥ 99.5%), cadmium dichloride (CdCl2·2.5H2O, AR ≥ 99.0%), nickel dichloride (NiCl2·6H2O, AR ≥ 98.0%), manganese dichloride (MnCl2·4H2O, AR ≥ 99.0%), zinc sulfate heptahydrate (ZnSO4·7H2O, AR ≥ 99.5%), cupric sulfate (CuSO4·5H2O, AR ≥ 99.0%), and potassium dichromate (K2Cr2O7, AR ≥ 99.8%) were purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Methyl mercury chloride was purchased from Ehrenstorfer GmbH (affiliation, Germany). The biochemical and hematological analysis were performed by Hitachi 7600-110 autoanalyzer (Hitachi Ltd, Tokyo, Japan) and blood analyzer (Sysmex XT-1800i; Sysmex Co, Kobe, Japan), respectively. Water maze testing was performed using a MWM video analysis system (RD1101-MWM-G, Shanghai Mobiledatum Information Technology Co., Ltd., China). The monoclonal antibodies including p38, P-p38, JNK, p-JNK (phosphorylated JNK), NFκB, Nrf2, Akt, and GAPDH were obtained from Cell Signaling Technology (Danfoss, MA, USA). Sprague Dawley (SD) rats were obtained from Zhejiang Provincial Laboratory Animal Science Center (Hangzhou, Zhejiang, China).
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6

Heavy Metal Toxicity Evaluation Protocol

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Lead acetate ((CH3COO)2Pb·3H2O, AR ≥ 99.5), cadmium dichloride (CdCl2·2.5H2O, AR ≥ 99.0), nickel dichloride (NiCl2·6H2O, AR ≥ 98.0), manganese dichloride (MnCl2·4H2O, AR ≥ 99.0), zinc sulfate heptahydrate (ZnSO4·7H2O, AR ≥ 99.5), cupric sulfate (CuSO4·5H2O, AR ≥ 99.0), and potassium dichromate (K2Cr2O7, AR ≥ 99.8), were all purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, People's Republic of China). Other reagents used include methyl mercury chloride which was purchased from Dr Ehrenstorfer GmbH (Germany). Biochemical analysis was performed using a Hitachi 7600-110 auto analyzer (Hitachi Ltd, Tokyo, Japan) and hematological analysis using a Sysmex blood analyser (Sysmex XT-1800i; Sysmex Co., Kobe, Japan). Sprague Dawley (SD) rats were obtained from the Zhejiang Provincial Laboratory Animal Science Center (Hangzhou, Zhejiang, People's Republic of China). Water maze testing was performed using a Morris water maze video analysis system (RD1101-MWM-G, Shanghai Mobiledatum Information Technology Co., Ltd, People's Republic of China).
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