The cells were plated in 12‐well plates at 1 × 105 density and incubated for 24 h. Subsequently, the cells were incubated with EIPA (50 µm ) or BTZ (20 µm ), in EBSS (plus 3% BSA) medium for 24 h. Then the cells were washed with PBS three times, collected, and stained with Annexin‐V/PI double staining kit (Beyotime, C1062S) according to protocol. Then the apoptosis was analyzed by Agilent NovoCyte Quanteon machine.
Annexin 5 pi double staining kit
Manufactured by Beyotime
Sourced in China
The Annexin-V/PI double staining kit is a laboratory tool used to detect and analyze cell apoptosis. It provides a fluorescent-based method to simultaneously stain cells with Annexin-V and Propidium Iodide (PI), which are commonly used markers for apoptosis and cell viability, respectively. The kit allows for the identification and quantification of early apoptotic, late apoptotic, and necrotic cell populations through flow cytometry or fluorescence microscopy analysis.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using annexin 5 pi double staining kit
1
Evaluating Apoptosis in Cell Lines
2
Annexin V/PI Apoptosis Assay in U87 Glioblastoma
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
An apoptosis assay was conducted using Annexin V/PI double-staining kit (Beyotime, Haimen, Jiangsu, China). Briefly, U87 glioblastoma cells were cultured in six-well plates at 37°C in an incubator overnight. The cells were treated with 10 and 20 µM Brevilin A for 24 hours. Following drug treatment, adherent and floating cells were collected, washed with PBS, and re-suspended in binding buffer. The samples were incubated with 5 µL Annexin V for 10 minutes in the dark, followed by incubation with 10 µL PI for another 15 minutes in the dark at room temperature according to the manufacturer’s instructions for the kit. After filtration, the cell samples were analyzed by flow cytometry (BD Accuri C6) for the detection of apoptotic cells.
3
Cell Cycle and Apoptosis Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were treated with the indicated doses of IR. After 24h, the cell cycle and apoptosis assay were determined. The cell cycle was analyzed using the Cell Cycle Kit (Beyotime) according to the manufacturer's instructions. Cultured cells were collected by trypsinization and washed twice in cold PBS. Then the cells fixed in 70% ethanol overnight at 4 °C and washed twice with cold PBS. Next, the cells were resuspended in RNase A/PI staining solution and incubated in the dark at 37 °C for 60 minutes. For apoptosis assays, floating and adherent cells were collected by trypsinization and washed twice with cold PBS. The cells were resuspended in 1×binding buffer at a concentration of 1×105 cells/mL, and stained for 15 minutes at room temperature in the dark using the AnnexinV/PI double staining Kit (Beyotime) according to the manufacturer's protocol. The cell cycle distribution and apoptosis rate were measured with flow cytometry (Becton-Dickinson, Mountain View, CA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!