Dfc450c

Manufactured by Leica

Sourced in Germany, Japan, Belgium, Switzerland

The DFC450C is a digital camera developed by Leica for use in microscopy and lab imaging applications. It features a high-resolution CMOS sensor and supports a range of imaging modes and resolutions suitable for various microscopy techniques.

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Lab products found in correlation

M165 fc, by Leica (11 mentions) M205 fa, by Leica (7 mentions) Dm il led, by Leica (7 mentions) Application suite software, by Leica (6 mentions) Las v 4, by Leica (5 mentions) Dm5500b, by Leica (4 mentions) Synergy htx, by Agilent Technologies (4 mentions) Lsm 880 confocal microscope, by Zeiss (4 mentions) Methylcellulose, by Thermo Fisher Scientific (3 mentions) Dm4000b microscope, by Leica (3 mentions) Dm2000, by Leica (3 mentions) Mz10f, by Leica (3 mentions) Dm6000b, by Leica (3 mentions) Rat acidic collagen extract, by Serva Electrophoresis (2 mentions) Medium 199, by Merck Group (2 mentions) Dm1000 led, by Leica (2 mentions) Tcs sp8, by Leica (2 mentions) Dm3000, by Leica (2 mentions) Szx16, by Leica (2 mentions) Confocal dish, by SPL Life Sciences (2 mentions)

Close spelling variants of this product

DFC450C microscope (15 citations) DFC450C RGB CCD camera (2 citations)

198 protocols using dfc450c

Analyzing Callose and ROS in Rice Leaves

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Rice sheaths were selected at the two-leaf stage and shortened to a length of 4 cm. The rice sheathes were then immersed in sterile water for 2 h and then placed in a Petri dish lined with wet filter paper. The purified BAS1 prokaryotic expression products were sprayed onto the leaves, which were then placed in an incubator at 26 °C and humidity for 24 h. The leaves were stained with aniline blue and DAB, respectively.
Callose observation: The leaves were soaked in ethanol lactophenol solution (12.5 g phenol, 12.5 mL glycerol, 12.5 mL lactic acid, and 12.5 mL water, mixed well) and kept in a 65 °C water bath until the chloroplasts were detached. The treated sheaths were rinsed with 50% ethanol, followed by rinsing with sterile water. The sheaths were then stained with 0.1% aniline blue (dissolved in 150 mmol/L K₂HPO₄, pH 9.5) for 0.5 h. The stained samples were then immersed in 50% glycerol. Callose deposition was observed with UV light under a fluorescent microscope (DFC450C, Leica).
ROS observation: After treatment with the protein solution, the leaves were then rinsed with sterile water, gently wiped with filter paper, and then stained with 1 mg/mL DAB solution for 24 h. The stained leaves were then soaked in ethanol lactophenol solution and kept in a 65 °C water bath until the chloroplasts were removed. ROS was observed under a fluorescent microscope (DFC450C, Leica).

Wang C., Liu Y., Liu L., Wang Y., Yan J., Wang C., Li C, & Yang J. (2019). The biotrophy-associated secreted protein 4 (BAS4) participates in the transition of Magnaporthe oryzae from the biotrophic to the necrotrophic phase. Saudi Journal of Biological Sciences, 26(4), 795-807.

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Quantifying Apoptosis via Fluorescence Microscopy

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Apoptosis was analysed by fluorescence microscopy (Leica, DFC450C). First, a total of 1.2 × 10⁴ cells were seeded onto 96‐well culture plates. After 24 hours, parental and PKM2 siRNA cells were treated with THP alone or combination, respectively. Another 24 hours later, 100 μL binding buffer, 1 μL of Annexin V‐FITC and 1 μL of propidium iodide were added into cells at room temperature in the dark for 15 minutes, kept in a 4°C temperature, and then examined and collected images under fluorescence microscopy. Images were taken by microscopy (Leica, DFC450C) and semi‐quantitative analysis with Image J; the average optical density indicates the fluorescence intensity; and the higher the average optical density, the more the number of apoptotic cells.

Su Q., Tao T., Tang L., Deng J., Darko K.O., Zhou S., Peng M., He S., Zeng Q., Chen A.F, & Yang X. (2018). Down‐regulation of PKM2 enhances anticancer efficiency of THP on bladder cancer. Journal of Cellular and Molecular Medicine, 22(5), 2774-2790.

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Microscopic Imaging of Cells at Low Temp

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The acquisition of images and time-lapse monitoring was performed using an inverted epifluorescence microscope (microscope: Leica DMI6000B, objective: Leica Microsystems HC PL APO 40x/0.85 CORR, camera: Leica DFC450C, software: LAS X 3.7) and an inverted confocal microscope (TCS SP5 AOBS, Leica Microsystems, camera: Leica DFC450C). The observations were made in the same lighting and temperature conditions as in the regular culture room. For this purpose, the microscope was placed in a room cooled to 17 °C and framed by a carbonate-glass chamber fitted to a thermostatically controlled air flow system (Model: CUBE & BOX, Life Imaging Services GmbH) to further cool the sample down to 13 °C. Two LED lamps were placed inside the chamber to provide adapted lighting (see Section 4.3 for detailed conditions).

Clerc T., Boscq S., Attia R., Kaminski Schierle G.S., Charrier B, & Läubli N.F. (2022). Cultivation and Imaging of S. latissima Embryo Monolayered Cell Sheets Inside Microfluidic Devices. Bioengineering, 9(11), 718.

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Characterization of Fabricated Transdermal Microneedles

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Microscopic images of the fabricated TA-DMNs were acquired using a bright field microscope (M165FC; Leica Camera AG, Wetzlar, Germany) and a digital microscope camera (DFC450C; Leica Camera AG) to assess the morphological properties of TA-DMNs. Morphological features of TA-DMNs, including the height and diameter of the tip and base, were measured using LAS v. 4.12 software. The mechanical strength of the TA-DMNs was assessed by measuring the minimal physical force required to create fractures using a force analyzer (Z0.5TN; Zwick Roell Inc., Ulm, Germany). The metal probe in the force analyzer descended at a speed of 2.0 mm/min to a single TA-DMN placed on the stage to measure the axial force needed to create a fracture in the TA-DMN.

Sim J., Kang G., Yang H., Jang M., Kim Y., Ahn H., Kim M, & Jung H. (2022). Development of Clinical Weekly-Dose Teriparatide Acetate Encapsulated Dissolving Microneedle Patch for Efficient Treatment of Osteoporosis. Polymers, 14(19), 4027.

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DMN Fracture Force Analysis

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Images of DMN patches were obtained using a stereomicroscope (M165FC, Leica Camera AG, Wetzlar, Germany) and a digital microscope camera (DFC450C, Leica Camera AG). The fracture force of the DMNs was analyzed using a force analyzer (Z0.5TN, Zwick Roell Inc., Ulm, Germany). A single DMN was placed on the test stage and a metal probe was moved downward at a continuous speed of 3.6 mm/min. After the probe reached the tip of the DMN, the axial force when the fracture of the DMN occurred was recorded as the fracture force.

Lee B.M., Lee C., Lahiji S.F., Jung U.W., Chung G, & Jung H. (2020). Dissolving Microneedles for Rapid and Painless Local Anesthesia. Pharmaceutics, 12(4), 366.

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Nuclear Morphology Analysis via Hoechst 33258

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Hoechst 33258 (Beyotime Institute of Biotechnology, Shanghai, China) was used to analyze nuclear morphology under fluorescence microscopy. Briefly, the treated cells were fixed with 4% paraformaldehyde for 10 min at room temperature and rinsed twice with PBS. Subsequently, the cells were incubated with Hoechst 33258 staining for 5 min. After washing twice with PBS, the cells were observed using a fluorescence microscope (Leica Microsystems CMS GmbH, DFC450 C, Wetzlar, Germany) at 350 nm excitation wavelength.

He J., Zhang A., Song Z., Guo S., Chen Y., Liu Z., Zhang J., Xu X., Liu J, & Chu L. (2019). The resistant effect of SIRT1 in oxidative stress-induced senescence of rat nucleus pulposus cell is regulated by Akt-FoxO1 pathway. Bioscience Reports, 39(5), BSR20190112.

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Sprouting Assay for HUVEC Spheroids

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As we previously described [15 (link)], 500 HUVECs in culture medium containing 20% methylcellulose (Thermo Fisher Scientific) were seeded in 96-well round bottom non-adherent plates and cultured for 24 h. Afterwards, spheroids were pelleted and resuspended in a polymerization solution, which was composed of 2 volumes basal medium containing 0.5% methylcellulose and 20% FCS and 1 volume collagen solution. The collagen solution was prepared with rat acidic collagen extract (Serva, Heidelberg, Germany), 10× Medium 199 (Sigma-Aldrich), and 0.2 M NaOH at a ratio of 8:1:1. Subsequently, a spheroid mixture (~50 spheroids) was placed into each well of a pre-warmed 24-well plate. After incubation for 45 min, the spheroids were exposed to different concentrations of brassinin for 24 h and then photographed by a phase-contrast microscope (DFC450C; Leica Microsystems, Wetzlar, Germany). Spheroid sprouting was quantified by measuring the cumulative sprout length, i.e., the overall length of all sprouts growing out of each spheroid, by utilizing the Leica LAS V4.8 software and expressed as a percentage of the vehicle-treated control group.

Gu Y., Becker V., Qiu M., Tang T., Ampofo E., Menger M.D, & Laschke M.W. (2022). Brassinin Promotes the Degradation of Tie2 and FGFR1 in Endothelial Cells and Inhibits Triple-Negative Breast Cancer Angiogenesis. Cancers, 14(14), 3540.

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Seed Coat Cell Morphometrics

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To measure seed coat cell size and number, the outer layer of the seed coats were freshly dissected from the seeds of ZB107 and ZB306, and soaked in ddH₂O in Petri dishes (60 × 15 mm). The samples were cut into small pieces (5 × 5 mm) and mounted on glass slides before examination by Leica microscope (DM5500B, Bensheim, Germany). Twenty to forty images from independent sections per seed were photographed with Leica Microsystems (DFC450C). The cell area and cell number of the seed coat were measured using Image J software.

Yu A., Wang Z., Zhang Y., Li F, & Liu A. (2019). Global Gene Expression of Seed Coat Tissues Reveals a Potential Mechanism of Regulating Seed Size Formation in Castor Bean. International Journal of Molecular Sciences, 20(6), 1282.

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Fluorescence Imaging of Infiltrated Leaves

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At the 4th day after the injection of the infiltration buffer, the lower surface of the infiltrated leaf was placed under a Leica fluorescent stereomicroscope (Leica Microsystems Ltd. DFC450 C). A region of 1 cm away from the original injection site was selected. This region was excited at a wavelength of 450–490 nm and the emission spectrum between 500 and 550 nm was recorded.

Li Y., Sun M., Wang X., Zhang Y.J., Da X.W., Jia L.Y., Pang H.L, & Feng H.Q. (2021). Effects of plant growth regulators on transient expression of foreign gene in Nicotiana benthamiana L. leaves. Bioresources and Bioprocessing, 8(1), 124.

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Cellular Morphology Observation Techniques

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When the cells converged about 80% on the coverslip, washing the slide with PBS solution. Cells were fixed with 4% paraformaldehyde for 20 min and washed three times with PBS, and staining nuclei with the hematoxylin solution for 8 min, and rinsing in running tap water. Then differentiating with 1% acid alcohol solution for 30 s, and rinsing in running tap water. Bluing in saturated lithium carbonate solution for 30 s, and rinsing in running tap water. After that, counterstaining in eosin solution for 1 min, and rinsing in running tap water.
Toluidine blue staining was also performed when cells were converged about 80%, washing the slide with PBS solution. Cells were fixed with 95% alcohol for 15 s and washed three times with PBS. Then staining with the toluidine blue solution for 5 min, and adding the equal volume distilled water for next 15 min. And rinsing in running tap water, drying by airing, glycerol sealing piece. Hematoxylin–eosin staining and toluidine blue staining were used to observe cellular morphology under an inverted microscope (Leica Microsystems CMS GmbH, DFC450 C, Wetzlar, Germany).

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