Triple toftm 5600 spectrometer

All chemicals in the studies were obtained from commercial sources and used without purification. [^99mTc]NaTcO₄ was eluted from a ⁹⁹Mo-^99mTc generator which was manufactured by Curium Netherlands B.V. (DRN 4329 Ultra-TechneKow FM., Westerduinweg, Netherlands). ESI-MS spectra were acquired by a Triple TOFTM 5600 spectrometer (AB Sciex, Singapore). The IR spectra were obtained by an IR-Affinity-1 spectrometer (Shimadzu, Kyoto, Japan). ¹H-NMR and ¹³C-NMR spectra were acquired on a JNM-ECS spectrophotometer (JEOL, Kyoto, Japan). Radioactivity was assessed using an HRS-1000 technetium analyser (Huaruison, Beijing, China) and a Wizard 2480 γ-counter (Perkin Elmer, Singapore). HPLC analysis was carried out with an analytical column (Kromasil, 100 A - 5 μm, 250 × 4.6 mm) and a SHIMADZU system (CL-20AVP, Kyoto, Japan) equipped with an SPD-20A UV detector (λ = 254 nm) and a Bioscan flow count 3200 NaI/PMT γ-radiation scintillation detector (Eckert&Zleger Radiopharma, Washington, DC, USA). SPECT/CT imaging was recorded on a Triumph SPECT/CT scanner (TriFoil Imaging, Los Angeles, CA, USA). Female Kunming mice (18–22 g) were obtained from Beijing Vital River Laboratory Animal Technology, Beijing, China.

The GXSTC, Choerospondias axillaris, Salvia miltiorrhiza Bunge, Syzigium aromaticum, Dryobalanops and Tabaschir were kindly provided by Buchang Pharmaceutical Co. Ltd. (Xi’an, China). The reference standards of gallic acid, danshensu, protocatechuic acid, protocatechuic aldehyde, ellagic acid, rosmarinic acid, salvianolic acid A/B/C, chlorogenic acid, eugenol, dihydrotanshinone I, cryptotanshinone, tanshinone I/IIA, caffeic acid, quercetin and miltionone were purchased from Nantong Feiyu Biotechnology Co. Ltd (Nantong, China). Their mass analysis was performed on the Triple TOF^TM 5600 spectrometer (AB SCIEX, Foster City, CA, USA) according to previous literature [18 (link),19 (link),20 ,21 (link),22 ,23 ]. Meanwhile, the purity of all standards was determined to be above 98% by LC analysis based on a peak area normalization method.
Acetonitrile and methanol (HPLC-grade) were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Formic acid (HPLC grade) and phosphoric acid (analytical grade) were supplied by Kemiou Chemical Reagent Co. Ltd. (Tianjin, China). Deionized water was purified by the Milli-Q water purification system from Millipore (Bedford, MA, USA). All other chemicals and reagents were analytical-grade and commercially available.