Beckman synchron lx20

According to the NHANES, a timed-endpoint method was used to measure serum iron level. Iron is released from transferrin by acetic acid and is reduced to the ferrous state. Then the ferrous ion is immediately complexed with the FerroZine Iron Reagent. The system monitors the changes in absorbance at 560 nm at a fixed-time interval and these changes in absorbance are proportional to the iron concentration in the samples. The Beckman Synchron LX20 or Beckman UniCel® DxC800 Synchron was used in 2003–2016, and the Roche Cobas 6000 (c501 module) analyzer was used in 2017–2018. Additionally, an enzymatic rate method or a kinetic rate method was used to measure the levels of serum ALT and AST using the Beckman Synchron LX20 or the Beckman UniCel® DxC800 Synchron in 2003–2018.

The principal variables of this study were sUA (independent variable) and total BMD (dependent variable). SUA levels were measured using a Hitachi Model 917 multichannel analyser (Roche Diagnostics, Indianapolis, IN) from 1999 to 2001 and a Beckman Synchron LX20 (Beckman Coulter, Inc., Brea, CA) in 2002. Distributions of UA results from the two laboratories were compared at the time of transition, and no significant differences were observed. Total BMD was measured by dual-energy X-ray absorptiometry.
In addition, the following covariates were included: age, sex, race/ethnicity, BMI, income-poverty ratio, physical activity, blood urea nitrogen, total protein, total cholesterol, serum phosphorus, and serum calcium. Details of sUA and total BMD measurement process and other covariate acquisition process are available at www.cdc.gov/nchs/nhanes/.

In-person interviews were used to collect demographic factors, such as age, gender, race, cigarette smoking status, and previous medical history. The race and ethnicity of participants were classified as Hispanic, non-Hispanic Black, non-Hispanic White, non-Hispanic Asian, and others. Participants were asked “Do you now smoke cigarettes?” to determine cigarette smoking use. Medical history was obtained from a self-reported questionnaire, including coronary heart disease, angina pectoris, and liver condition. Body mass index (BMI) was estimated by dividing the weight (kg) by the square of height (m) (kg/m²). Height was estimated using a portal stadiometer, a digital measurement device connected to the acrylic headpiece and interfaced directly with the NHANES Integrated Survey Information System (ISIS) anthropometry application. Weight was estimated using a portal digital weight scale, which was a high-performance instrument linked directly to the ISIS anthropometry application. Serum calcium concentration was analyzed using Beckman Synchron LX20 (Beckman Coulter, Brea, CA). Laboratory biochemical data included glucose, alanine aminotransferase, platelet, creatinine, high density-lipoprotein cholesterol, and C-reactive protein, which were collected by trained medical technologists using standard procedures.

Blood was obtained through capillary blood collection; fasting blood glucose (FBG) was examined instantly using the One-Touch FreeStyle Freedom Lite blood glucose monitor (Abbott Diabetes Car Inc., CA, USA). Blood biomarkers, such as insulin, triglycerides, total cholesterol (TC), aspartate transaminase (AST), alanine transaminase (ALT), creatinine, blood urea nitrogen (BUN), uric acid, high-sensitivity C-reactive protein (hs-CRP), and LDL-C, and high-density lipoprotein-cholesterol (HDL-C), were analyzed from a 4-mL blood sample by using the Beckman Synchron LX-20 (Beckman Coulter Inc., CA, USA). Glycated hemoglobin (HbA1c) levels were analyzed from another 1.5-mL blood sample by using Spotchem SP-4410 (Arkray Inc., Kyoto, Japan). All the listed experiments and procedures were performed in the authorized Yea-Tong laboratory center, in Chung-Li City, Taiwan.