Freshly harvested spikelets belonging to 7 DAP harvested from SN and LGR were immersed completely in FAE fixative solution [10% formaldehyde, 5% acetic acid, and 50% absolute ethanol] in 15 ml Borosil® glass vials. These samples were vacuum infiltrated for 30 min and incubated at 4 °C overnight in dark. The tissue samples were subjected to dehydration series with increasing gradient of ethanol (60%, 70%, 80%, 95%, and 100%) at room temperature for 1 h. The outer surface cells of lemma were observed under EVO® LS10 scanning electron microscope (ZEISS, Germany) at × 500 magnifications. The images were analyzed using ImageJ [175 (link)] to estimate cell length and cell area of the outer epidermal cells in the middle region of the lemma.
Evo ls10 scanning electron microscope
Manufactured by Zeiss
Sourced in Germany
The EVO® LS10 is a scanning electron microscope (SEM) manufactured by Zeiss. It is designed to provide high-resolution imaging and analysis of a wide range of sample types. The EVO® LS10 utilizes an electron beam to scan the surface of a sample, generating detailed images and data about the sample's topography, composition, and other characteristics.
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Lab products found in correlation
5 protocols using evo ls10 scanning electron microscope
1
Lemma Epidermal Cell Morphometrics
2
SEM Examination of Materials
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Glass coverslips and the bottoms of the polystyrene bijou containers sent for SEM examination were firstly fixed in 2,5% glutaraldehyde in 0.1 M Sorensen phosphate buffer. After several rinses in the same buffer solution, the different materials were dehydrated with a series of alcohol solutions at increasing concentrations. Then, they were subjected to the critical point dryer procedure by means of a CPD 030 apparatus (BAL-TEC AG, Balzers, Liechtestein), metallized with a 3 nm gold layer in a SCD 050 apparatus (BAL-TEC AG, Balzers, Liechtestein) and examined by means of a EVO LS 10 scanning electron microscope (Zeiss, Oberkochen, Germany) operating at a voltage acceleration of 20 kV.
3
Visualizing C. perfringens J21 by SEM
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C. perfringens J21 was grown to mid-log phase (OD600 = 0.4–0.6) and cells washed with PBS buffer and suspended to OD600 = 0.6. LysCP28 was added (final concentration 10 μg/mL) to the culture and incubated at 37 °C for 30 min. The bacterial mixtures were sampled at 0, 10, 20, and 30 min and centrifuged 3000× g for 5 min at 4 °C. The pellets were treated with precooled 2.5% glutaraldehyde solution and fixed at 4 °C for 2–12 h. The samples were then washed twice with 10 mM sodium phosphate (pH 7.5) and dehydrated in 50, 70, 90, and 99.5% ethanol. Subsequently, the ethanol was replaced with 50% ethanol−50% isoamyl acetate and 100% isoamyl acetate. The samples were dried using a critical-point dryer and subsequently sputter-coated with platinum using an ion sputtering machine. The grid was then loaded into a ZEISS EVO-LS10 scanning electron microscope (ZEISS, Germany), and the bacteria were examined at a 5000× magnification.
4
Pollen Fertility Evaluation in Rice
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The materials planted at the Sanya breeding base (Sanya, Hainan) and Xishuangbanna breeding base (Jinghong, Yunnan) were investigated for the indicated times. During heading, florets in the upper middle section of flowering primary panicles, twenty per plant, were sampled and fixed with 75% alcohol. Anthers of three florets were randomly selected from the sampled florets and squashed in 0.01 g/mL iodine and iodine-potassium solution (I2-KI) for staining the pollen grains. The pollen grains that were round, dark and uniformly stained were considered fertile. In contrast, the pollen grains that exhibited unstained spherical or shriveled shapes or pale coloration were considered sterile. Investigation of pollen fertility was carried out under a Carl Zeiss microscope (Carl Zeiss, Germany) at 10 × magnification. The pollen fertility of each plant was measured for three repetitions. Moreover, the morphology of anthers and pollen grains was photographed by an OLYMPUS SZX16 stereoscope microscope (Olympus, Japan) and a ZEISS EVO LS10 scanning electron microscope (Carl Zeiss, Germany), respectively.
5
Ultrastructural Analysis of Leishmania Parasites
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Scanning electron microscopy (SEM) was used for morphological analysis of promastigotes and axenic amastigotes. Samples were fixed by using 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer overnight at 4 °C and postfixed in 1% osmium tetroxide. Thereafter, they were washed with sodium cacodylate buffer (pH 7.3), adhered on poly-L-lysine-coated coverslips, and dehydrated in an ascending series of ethanol. The samples were critical-point dried in CO2, coated with gold, and observed in a ZEISS EVO LS10 scanning electron microscope (Zeiss, Jena, Germany).
Transmission electron microscopy was used for ultrastructural analysis. After washing with 1× PBS, samples were fixed by using 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.3) overnight at 4 °C and postfixed in 1% osmium tetroxide. The parasites were dehydrated in an acetone series and embedded in araldite resin for 72 h at 60 °C. Staining of ultrathin sections of the embedded resin was done by using 5% uranyl acetate and lead citrate. Finally, the sections were examined in a JEOL JEM-2100 F/HR transmission electron microscope (Jeol, Tokyo, Japan).
Transmission electron microscopy was used for ultrastructural analysis. After washing with 1× PBS, samples were fixed by using 2.5% glutaraldehyde in 0.1 M sodium cacodylate buffer (pH 7.3) overnight at 4 °C and postfixed in 1% osmium tetroxide. The parasites were dehydrated in an acetone series and embedded in araldite resin for 72 h at 60 °C. Staining of ultrathin sections of the embedded resin was done by using 5% uranyl acetate and lead citrate. Finally, the sections were examined in a JEOL JEM-2100 F/HR transmission electron microscope (Jeol, Tokyo, Japan).
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