RNA was isolated using the Ambion® RNaqueous® micro kit (ThermoFisher Scientific) according to manufacturer’s instructions. RNA quality was analyzed using the Agilent 6000 Pico LabChip® and Agilent 2100 Bioanalyzer software (Agilent Technologies, USA). RNA quantity was determined using the Quant-iT™ RiboGreen® kit (ThermoFisher Scientific) according to manufacturer’s instructions. NEBNext Oligo d(T)25 beads (New England BioLabs, Ipswich, MA, USA) were used for mRNA purification. cDNA library synthesis was performed according to Ziegler et al.64 (link) with three biological replicates per treatment each containing three A. thaliana leaves. Subsequently, 100 bp paired-end RNA sequencing was performed on the Illumina HiSeq4000 platform (Génome Québec Innovation Centre, McGill University, Montreal, Canada).
Agilent 6000 pico labchip
Manufactured by Agilent Technologies
Sourced in United States
The Agilent 6000 Pico LabChip is a compact, automated microfluidic system designed for high-throughput sample analysis. It enables rapid and precise separation, detection, and quantitation of various biomolecules, including proteins, nucleic acids, and cell samples. The system integrates sample handling, separation, and detection functionalities on a single platform, providing efficient and reliable analytical capabilities.
Automatically generated - may contain errors
Lab products found in correlation
Agilent 2100 bioanalyzer, by Agilent Technologies (3 mentions)
Hiseq 2000 platform, by Illumina (2 mentions)
Quant it ribogreen kit, by Thermo Fisher Scientific (1 mentions)
Nebnext oligo dt 25 beads, by New England Biolabs (1 mentions)
Ambion rnaqueous micro kit, by Thermo Fisher Scientific (1 mentions)
Hiseq 4000 platform, by Illumina (1 mentions)
3 protocols using agilent 6000 pico labchip
1
Transcriptomic Analysis of Arabidopsis Leaves
2
RNA-seq Library Preparation and Sequencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
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RNA was isolated using the Ambion ® RNaqueous ® micro kit according to manufacturer's instructions. RNA quality was analyzed using the Agilent 6000 Pico LabChip ® and Agilent 2100 Bioanalyzer software (Agilent Technologies, USA). RNA quantity was determined using the Quant-iT™ RiboGreen ® kit according to manufacturer's instructions. cDNA library synthesis was performed according to Ziegler et al. (2019) . Subsequently, 100 bp single-end RNA sequencing was performed using the Illumina HiSeq2000 platform (Génome Québec Innovation Centre, McGill University, Montreal, Canada).
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
3
RNA-seq Library Preparation and Sequencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
RNA was isolated using the Ambion ® RNaqueous ® micro kit according to manufacturer's instructions. RNA quality was analyzed using the Agilent 6000 Pico LabChip ® and Agilent 2100 Bioanalyzer software (Agilent Technologies, USA). RNA quantity was determined using the Quant-iT™ RiboGreen ® kit according to manufacturer's instructions. cDNA library synthesis was performed according to Ziegler et al. (2019) . Subsequently, 100 bp single-end RNA sequencing was performed using the Illumina HiSeq2000 platform (Génome Québec Innovation Centre, McGill University, Montreal, Canada).
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
(which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
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